Protein Info for EX28DRAFT_3721 in Enterobacter asburiae PDN3

Annotation: membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 796 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 41 to 58 (18 residues), see Phobius details amino acids 65 to 81 (17 residues), see Phobius details amino acids 87 to 90 (4 residues), see Phobius details amino acids 94 to 113 (20 residues), see Phobius details amino acids 120 to 140 (21 residues), see Phobius details TIGR03074: membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family" amino acids 15 to 792 (778 residues), 1182.9 bits, see alignment E=0 PF13360: PQQ_2" amino acids 220 to 309 (90 residues), 20.5 bits, see alignment E=4.9e-08 amino acids 670 to 775 (106 residues), 22.8 bits, see alignment E=9.9e-09 PF01011: PQQ" amino acids 225 to 259 (35 residues), 34.9 bits, see alignment (E = 1.3e-12) amino acids 292 to 320 (29 residues), 23.1 bits, see alignment (E = 7.2e-09) amino acids 362 to 388 (27 residues), 23 bits, see alignment (E = 7.3e-09) amino acids 443 to 467 (25 residues), 26.3 bits, see alignment (E = 6.8e-10) amino acids 663 to 697 (35 residues), 25.4 bits, see alignment (E = 1.3e-09) amino acids 721 to 758 (38 residues), 26.8 bits, see alignment 4.6e-10

Best Hits

Swiss-Prot: 90% identical to DHG_ECOLI: Quinoprotein glucose dehydrogenase (gcd) from Escherichia coli (strain K12)

KEGG orthology group: K00117, quinoprotein glucose dehydrogenase [EC: 1.1.5.2] (inferred from 97% identity to enc:ECL_00929)

MetaCyc: 90% identical to quinoprotein glucose dehydrogenase (Escherichia coli K-12 substr. MG1655)
Quinoprotein glucose dehydrogenase. [EC: 1.1.5.2]

Predicted SEED Role

"Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2)" in subsystem D-gluconate and ketogluconates metabolism or Entner-Doudoroff Pathway or Respiratory dehydrogenases 1 (EC 1.1.5.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.5.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (796 amino acids)

>EX28DRAFT_3721 membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family (Enterobacter asburiae PDN3)
MAETNSKQPRLLVTLTAAFAAFCALYLLIGGVWLVALGGSWYYPIAGLVMVAVTVLLLRR
KQSALWLYAALLLATMIWGVWEVGFDFWALTPRSDILVFFGIWLILPFVWRRLIVPSSGA
VAGLVVALLITGGILTWAGFNDPQEINGTLNAESTPAAAISQVADGDWPAYGRNQEGQRY
SPLKQINADNVKNLKEAWVFRTGDVKMPNDPGELTNEVTPIKVGNMLYLCTAHQRLFALD
AATGKEKWHFDPQLNSNPSFQHVTCRGVSYHEARAENASPEVVADCPRRIMLPVNDGRLF
AINAETGKLCETFANKGILNLQTNMPDTTPGLYEPTSPPIITDKTIVIAGSVTDNFSTRE
TSGVIRGFDVNTGKLLWAFDPGAKDPNAIPSDEHTFTFNSPNSWAPAAYDAKLDLVYLPM
GVTTPDIWGGNRTPEQERYASAIVALNATTGKLAWSYQTVHHDLWDMDMPSQPTLADITV
DGKTVPVIYAPAKTGNIFVLDRSNGKLVVPAPEKPVPQGAAKGDYVSKTQPFSDLSFRPK
KDLSGADMWGATMFDQLVCRVMFHQLRYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPN
RQVAIANPMALPFVSRLIPRGPGNPMEQPKDAKGSGTEAGIQPQYGVPFGVTLNPFLSPF
GLPCKQPAWGYISGLDLKTNQIVWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAG
NVLFIAATADNYLRAYNMTNGEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGT
KMGDYIVAYALPDENK