Protein Info for EX28DRAFT_3720 in Enterobacter asburiae PDN3

Annotation: Putative multicopper oxidases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 535 signal peptide" amino acids 1 to 42 (42 residues), see Phobius details PF10518: TAT_signal" amino acids 13 to 33 (21 residues), 23.2 bits, see alignment (E = 9.4e-09) PF07732: Cu-oxidase_3" amino acids 65 to 179 (115 residues), 108.1 bits, see alignment E=6.1e-35 PF07731: Cu-oxidase_2" amino acids 423 to 535 (113 residues), 84.1 bits, see alignment E=1.6e-27

Best Hits

Swiss-Prot: 81% identical to CUEO_SALTY: Blue copper oxidase CueO (cueO) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K14588, blue copper oxidase (inferred from 92% identity to enc:ECL_00928)

MetaCyc: 82% identical to multicopper oxidase CueO (Escherichia coli K-12 substr. MG1655)
RXN0-2945 [EC: 1.16.3.4]; Ferroxidase. [EC: 1.16.3.4, 1.16.3.1]; Laccase. [EC: 1.16.3.4, 1.16.3.1, 1.10.3.2]; RXN0-2943 [EC: 1.16.3.4, 1.16.3.1, 1.10.3.2]

Predicted SEED Role

"Blue copper oxidase CueO precursor" in subsystem Copper homeostasis

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.16.3.1

Use Curated BLAST to search for 1.10.3.2 or 1.16.3.1 or 1.16.3.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (535 amino acids)

>EX28DRAFT_3720 Putative multicopper oxidases (Enterobacter asburiae PDN3)
MLKRSPAGNKEMNMQRRDFLKYSVVLGAASALPLWSRAAFAADRPALPIPDLVTADARSR
IQLVVQSGKTTFGANTATTWGYNGNLLGPALQLRKGKTVTVDIHNTLSEETTLHWHGLEV
PGEVDGGPQGIIKAGGTRSVTFTPDQRAATCWFHPHQHGKTGHQVAMGLAGLVLIEDDES
RLLRLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMSAAVGWFGDTLLTNGAIYPQHAAP
KGWLRLRLLNGCNARSLNFAASDKRPLYVVASDGGLLPEPVKVSELPMLMGERFEVLVDI
SDGKPFDLVTLPVSQMGMAVAPFDKPHPVLRIQPLLVAASGTLPDTLTTLPALPSLEGLT
QRRLQLSMNPMLDMMGMQALMAKYGDKAMAGMQHGQMMGHMNMDHGKMGGMGNMNHGDHG
FDFHNANMINGKAFDMNTPMFAATKGQFERWVISGEGDMMLHPFHTHGTQFRILTENGKA
PDAHRAGWKDTVRVEGGVSEVLVKFDHEAPKEFAYMAHCHLLEHEDTGMMLGFTV