Protein Info for EX28DRAFT_3711 in Enterobacter asburiae PDN3

Annotation: pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase, long form

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00364: Biotin_lipoyl
  • amino acids 4 to 73 (70 residues), 76.8 bits, E=2.9e-25
  • amino acids 106 to 176 (71 residues), 79.1 bits, E=5.2e-26
  • amino acids 208 to 277 (70 residues), 78.2 bits, E=9.9e-26
• TIGR01348: dihydrolipoyllysine-residue acetyltransferase
  • amino acids 190 to 631 (442 residues), 753.6 bits, E=7.3e-231
• PF02817: E3_binding
  • amino acids 327 to 362 (36 residues), 58.8 bits, 1.5e-19
• PF00198: 2-oxoacid_dh
  • amino acids 400 to 630 (231 residues), 270.3 bits, E=4.1e-84

Best Hits

Swiss-Prot: 90% identical to ODP2_ECOLI: Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (aceF) from Escherichia coli (strain K12)

KEGG orthology group: K00627, pyruvate dehydrogenase E2 component (dihydrolipoamide acetyltransferase) [EC: 2.3.1.12] (inferred from 98% identity to enc:ECL_00914)

MetaCyc: 90% identical to pyruvate dehydrogenase E2 subunit (Escherichia coli K-12 substr. MG1655)
Dihydrolipoyllysine-residue acetyltransferase. [EC: 1.2.1.104, 2.3.1.12]; 1.2.1.104 [EC: 1.2.1.104, 2.3.1.12]

Predicted SEED Role

"Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 2.3.1.12)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
• pyruvate fermentation to acetate II (3/3 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (3/4 steps found)
• pyruvate fermentation to acetate V (2/3 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (5/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (10/17 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glycolysis / Gluconeogenesis
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.104 or 2.3.1.12

Protein Sequence (631 amino acids)

>EX28DRAFT_3711 pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase, long form (Enterobacter asburiae PDN3)
MAIEINVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGIVKEIKVS
VGDKTETGKLIMIFDSADGAAAAAPAQEEKKAAPAAAAPAAAASAKEVNVPDIGGDEVEV
TEILVKVGDTVAAEQSLITVEGDKASMEVPAPFAGTVKEIKINTGDKVSTGSLIMVFEVA
GAAPAAAPAQAAAPAPAAAPAAAGGAKDVNVPDIGGDEVEVTEVMVKVGDKVAAEQSLIT
VEGDKASMEVPAPFAGTVKEIKISTGDKVSTGSLIMVFEVEGAAPAAAPAAAAAPAPAAA
PAQAAKPAAAPAAKAEKSEFAENDAYVHATPLIRRLAREFGVNLAKVKGTGRKGRILRED
VQTYVKDAVKRAEAAPAAAAGGGIPGMLPWPKVDFSKFGEIEEVELGRIQKISGANLSRN
WVMIPHVTHFDKTDITDLEAFRKQQNAEAEKRKLDVKFTPVVFIMKAVAAALEQMPRFNS
SLSEDGQKLTLKKYINIGVAVDTPNGLVVPVFKDVNKKSITELSRELTVISKKARDGKLT
AGEMQGGCFTISSIGGLGTTHFAPIVNAPEVAILGVSKSAMEPVWNGKEFVPRLMMPISL
SFDHRVIDGADGARFITIINNMLSDIRRLVM