Protein Info for EX28DRAFT_3703 in Enterobacter asburiae PDN3

Annotation: N-acetylmuramoyl-L-alanine amidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01510: Amidase_2
  • amino acids 26 to 167 (142 residues), 115.6 bits, E=1.1e-37

Best Hits

Swiss-Prot: 92% identical to AMPD_ENTCL: 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD (ampD) from Enterobacter cloacae

KEGG orthology group: K03806, AmpD protein (inferred from 92% identity to enc:ECL_00906)

MetaCyc: 86% identical to 1,6-anhydro-N-acetylmuramoyl-L-alanine amidase (Escherichia coli K-12 substr. MG1655)
N-acetylmuramoyl-L-alanine amidase. [EC: 3.5.1.28]

Predicted SEED Role

"N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) AmpD" (EC 3.5.1.28)

MetaCyc Pathways

• peptidoglycan recycling I (14/14 steps found)
• peptidoglycan recycling II (7/10 steps found)

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.28

Use Curated BLAST to search for 3.5.1.28

Protein Sequence (183 amino acids)

>EX28DRAFT_3703 N-acetylmuramoyl-L-alanine amidase (Enterobacter asburiae PDN3)
MLLENGWLVDARRVPSPHHDCRPEDEKPTLLVVHNISLPPGEFGGPWIDALFTGTIDPDA
HPFFAEIAHLRVSAHCLIRRDGEVVQYVPFDKRAWHAGVSMYHGRERCNDFSIGIELEGT
DTTPYTDSQYQQLAAVTRTLIGLYPAIADNITGHSDIAPERKTDPGPAFDWPRFRAMLTA
SSE