Protein Info for EX28DRAFT_3682 in Enterobacter asburiae PDN3

Annotation: Phospho-N-acetylmuramoyl-pentapeptide-transferase (EC 2.7.8.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 360 transmembrane" amino acids 19 to 40 (22 residues), see Phobius details amino acids 73 to 90 (18 residues), see Phobius details amino acids 96 to 114 (19 residues), see Phobius details amino acids 134 to 152 (19 residues), see Phobius details amino acids 172 to 192 (21 residues), see Phobius details amino acids 200 to 219 (20 residues), see Phobius details amino acids 239 to 256 (18 residues), see Phobius details amino acids 263 to 284 (22 residues), see Phobius details amino acids 290 to 311 (22 residues), see Phobius details amino acids 338 to 357 (20 residues), see Phobius details TIGR00445: phospho-N-acetylmuramoyl-pentapeptide-transferase" amino acids 37 to 360 (324 residues), 536.8 bits, see alignment E=1.1e-165 PF10555: MraY_sig1" amino acids 68 to 80 (13 residues), 24.1 bits, see alignment (E = 1.9e-09) PF00953: Glycos_transf_4" amino acids 99 to 284 (186 residues), 116.6 bits, see alignment E=1.1e-37

Best Hits

Swiss-Prot: 99% identical to MRAY_ENT38: Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY) from Enterobacter sp. (strain 638)

KEGG orthology group: K01000, phospho-N-acetylmuramoyl-pentapeptide-transferase [EC: 2.7.8.13] (inferred from 98% identity to ecd:ECDH10B_0069)

MetaCyc: 98% identical to phospho-N-acetylmuramoyl-pentapeptide-transferase (Escherichia coli K-12 substr. MG1655)
Phospho-N-acetylmuramoyl-pentapeptide-transferase. [EC: 2.7.8.13]

Predicted SEED Role

"Phospho-N-acetylmuramoyl-pentapeptide-transferase (EC 2.7.8.13)" in subsystem Peptidoglycan Biosynthesis (EC 2.7.8.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.8.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (360 amino acids)

>EX28DRAFT_3682 Phospho-N-acetylmuramoyl-pentapeptide-transferase (EC 2.7.8.13) (Enterobacter asburiae PDN3)
MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRMIARLQKLSFGQVVRND
GPESHFSKRGTPTMGGIMILTAIVVSVLLWAYPSNPYVWCVLTVLVGYGIIGFVDDYRKV
VRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATELVVPFFKDVMPQLGLFYILLA
YFVIVGTGNAVNLTDGLDGLAIMPTVFVAAGFALVAWATGNMNFANYLHIPYLRHAGELV
IVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGALGIIAVLLRQEFLLVIMGGVFVVE
TLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWPEPRVIVRFWIISLMLVLIGLATLKVR