Protein Info for EX28DRAFT_3677 in Enterobacter asburiae PDN3
Annotation: 16S rRNA (cytosine(1402)-N(4))-methyltransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 94% identical to RSMH_KLEP3: Ribosomal RNA small subunit methyltransferase H (rsmH) from Klebsiella pneumoniae (strain 342)
KEGG orthology group: K03438, S-adenosyl-methyltransferase [EC: 2.1.1.-] (inferred from 98% identity to enc:ECL_00879)MetaCyc: 94% identical to 16S rRNA m4C1402 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11638 [EC: 2.1.1.199]
Predicted SEED Role
"rRNA small subunit methyltransferase H" in subsystem Bacterial Cell Division
KEGG Metabolic Maps
- Alkaloid biosynthesis I
- Anthocyanin biosynthesis
- Benzoxazinone biosynthesis
- Biosynthesis of alkaloids derived from shikimate pathway
- Carotenoid biosynthesis - General
- Flavonoid biosynthesis
- Histidine metabolism
- Insect hormone biosynthesis
- Naphthalene and anthracene degradation
- Phenylpropanoid biosynthesis
- Porphyrin and chlorophyll metabolism
- Tryptophan metabolism
- Tyrosine metabolism
- Ubiquinone and menaquinone biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.1.1.-
Use Curated BLAST to search for 2.1.1.- or 2.1.1.199
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (313 amino acids)
>EX28DRAFT_3677 16S rRNA (cytosine(1402)-N(4))-methyltransferase (Enterobacter asburiae PDN3) MMENYKHKTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQLGEEGRLLAIDRDPQA IAVAQTINDPRFSIVHGPFSALAEYVAERGLTGKIDGILLDLGVSSPQLDDAERGFSFMR DGPLDMRMDPTRGQSAAEWLQTADEADIAWVIKTFGEERFGKRIARAIVERNRIEPMTRT KELAEVIAAATPVKDKHKHPATRTFQAVRIWVNSELEEIELALKSSLDVLAPGGRLSIIS FHSLEDRIVKRFMREQSRGPQVPAGLPMTEEQLRKLGGRQLRALGKLMPGEEEVAENPRA RSSVLRVAERTNA