Protein Info for EX28DRAFT_3628 in Enterobacter asburiae PDN3

Annotation: Na+/H+ antiporter NhaA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 391 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 59 to 77 (19 residues), see Phobius details amino acids 95 to 115 (21 residues), see Phobius details amino acids 126 to 145 (20 residues), see Phobius details amino acids 154 to 175 (22 residues), see Phobius details amino acids 181 to 200 (20 residues), see Phobius details amino acids 207 to 237 (31 residues), see Phobius details amino acids 257 to 279 (23 residues), see Phobius details amino acids 288 to 312 (25 residues), see Phobius details amino acids 324 to 350 (27 residues), see Phobius details amino acids 357 to 380 (24 residues), see Phobius details PF06965: Na_H_antiport_1" amino acids 6 to 380 (375 residues), 493.2 bits, see alignment E=2.3e-152 TIGR00773: Na+/H+ antiporter NhaA" amino acids 7 to 380 (374 residues), 600.1 bits, see alignment E=8.2e-185

Best Hits

Swiss-Prot: 90% identical to NHAA_ENT38: Na(+)/H(+) antiporter NhaA (nhaA) from Enterobacter sp. (strain 638)

KEGG orthology group: K03313, Na+:H+ antiporter, NhaA family (inferred from 96% identity to enc:ECL_00829)

MetaCyc: 80% identical to Na+:H+ antiporter NhaA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-129; TRANS-RXN-292

Predicted SEED Role

"Na+/H+ antiporter NhaA type" in subsystem Na(+) H(+) antiporter

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (391 amino acids)

>EX28DRAFT_3628 Na+/H+ antiporter NhaA (Enterobacter asburiae PDN3)
MKLLHRFFSSEASGGVILIIAAAAAMVLANLGITQELYHAFLETPVELKVGALEINKNML
LWINDALMAVFFLLVGLEVKRELVLGSLASRQRAAFPVIAAIGGMVVPALLFLAFTWQDP
VARDGWAIPAATDIAFALGVLALLGSRVPVALKIFLMALAIIDDLGAIVIIALFYTSDLS
ILSLSVAAGAIAVLALLNIFNVRRIGIYILVGMVLWTAVLKSGVHATLAGVIVGFFVPLK
PQDGKSPAKQLEHVLHPWVAFMILPLFAFANAGVSLGGVTLDGLTSVLPLGIIAGLFIGK
PLGISLFCWLATKLKLASLPHGTTFGQIMAVGVLCGIGFTMSIFISTLAFGAHAPELIVW
AKLGILIGSLLAAVIGYTLLKVKLSGQAVQA