Protein Info for EX28DRAFT_3625 in Enterobacter asburiae PDN3

Annotation: chaperone protein DnaK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 637 TIGR02350: chaperone protein DnaK" amino acids 3 to 603 (601 residues), 966.8 bits, see alignment E=2e-295 PF00012: HSP70" amino acids 4 to 602 (599 residues), 896.9 bits, see alignment E=5.6e-274 PF06723: MreB_Mbl" amino acids 116 to 376 (261 residues), 46.8 bits, see alignment E=2e-16

Best Hits

Swiss-Prot: 97% identical to DNAK_KLEP3: Chaperone protein DnaK (dnaK) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: K04043, molecular chaperone DnaK (inferred from 98% identity to enc:ECL_00827)

MetaCyc: 94% identical to chaperone protein DnaK (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein DnaK" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (637 amino acids)

>EX28DRAFT_3625 chaperone protein DnaK (Enterobacter asburiae PDN3)
MGKIIGIDLGTTNSCVAIMDGTTARVLENAEGDRTTPSIIAYTQDGETLVGQPAKRQAVT
NPQNTLFAIKRLIGRRFQDEEVQRDVSIMPYKIIAADNGDAWLDVKGAKTAPPQISAEVL
KKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAYG
LDKEVGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEVLATNGDTHLGGEDFDTRLINYL
VDEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHMNIKV
TRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDIQDVILVGGQTRMPMVQKKVAEFFGKE
PRKDVNPDEAVAIGAAVQGGVLTGEVKDVLLLDVTPLSLGIETMGGVMTALINKNTTIPT
KHSQVFSTAEDNQSAVTIHVIQGERKRAADNKSLGQFNLDGINPAPRGMPQIEVTFDIDA
DGILHVSAKDKNSGKEQKITIKASSGLNEAEIEKMVRDAEANAESDRKFEELVQTRNQGD
HLLHSTRKQVEEAGDKLPAEDKTAIEAALSALESSLKGEDKADIEAKMQELAQASQKLME
IAQQQHAQQQAGADASANNAKDDDVVDAEFEEVKDKK