Protein Info for EX28DRAFT_3597 in Enterobacter asburiae PDN3

Annotation: DNA repair protein RadA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 461 TIGR00416: DNA repair protein RadA" amino acids 1 to 455 (455 residues), 782.6 bits, see alignment E=5.5e-240 PF18073: Rubredoxin_2" amino acids 9 to 36 (28 residues), 43.3 bits, see alignment (E = 7e-15) PF06745: ATPase" amino acids 79 to 149 (71 residues), 42.3 bits, see alignment E=1.8e-14 PF13481: AAA_25" amino acids 90 to 226 (137 residues), 53.1 bits, see alignment E=9.5e-18 PF13541: ChlI" amino acids 345 to 434 (90 residues), 37.7 bits, see alignment E=5.4e-13 PF05362: Lon_C" amino acids 357 to 456 (100 residues), 32.6 bits, see alignment E=1.8e-11

Best Hits

Swiss-Prot: 96% identical to RADA_ECOLI: DNA repair protein RadA (radA) from Escherichia coli (strain K12)

KEGG orthology group: K04485, DNA repair protein RadA/Sms (inferred from 99% identity to enc:ECL_00800)

Predicted SEED Role

"DNA repair protein RadA" in subsystem DNA repair, bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (461 amino acids)

>EX28DRAFT_3597 DNA repair protein RadA (Enterobacter asburiae PDN3)
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRGVAASPSVARNERLSGYAGNAG
VAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKL
AEQMKTLYVTGEESLQQVAMRAHRLGLPTGNLNMLSETSIEQICMIAEEEQPKLMVIDSI
QVMHMADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDC
SVMLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEITSGSS
VMVLWEGTRPLLVEIQALVDVSMMGNPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVF
VNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEVGLAGEIRPVPSGQERISEA
AKHGFRRAIVPAANVPKKIPEGMQVFGVKKLADALNVFDDL