Protein Info for EX28DRAFT_3593 in Enterobacter asburiae PDN3

Annotation: purine-nucleoside phosphorylase, family 1 (deoD)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00107: purine nucleoside phosphorylase
  • amino acids 5 to 235 (231 residues), 408.8 bits, E=3.1e-127
• PF01048: PNP_UDP_1
  • amino acids 16 to 224 (209 residues), 136 bits, E=7.3e-44

Best Hits

Swiss-Prot: 98% identical to DEOD_ENT38: Purine nucleoside phosphorylase DeoD-type (deoD) from Enterobacter sp. (strain 638)

KEGG orthology group: K03784, purine-nucleoside phosphorylase [EC: 2.4.2.1] (inferred from 97% identity to esc:Entcl_3748)

MetaCyc: 94% identical to purine nucleoside phosphorylase (Escherichia coli K-12 substr. MG1655)
Purine-nucleoside phosphorylase. [EC: 2.4.2.1, 2.4.2.15]; 2.4.2.1 [EC: 2.4.2.1, 2.4.2.15]; 2.4.2.1 [EC: 2.4.2.1, 2.4.2.15]; 2.4.2.1 [EC: 2.4.2.1, 2.4.2.15]; 2.4.2.1 [EC: 2.4.2.1, 2.4.2.15]; 2.4.2.1 [EC: 2.4.2.1, 2.4.2.15]; 2.4.2.1 [EC: 2.4.2.1, 2.4.2.15]

Predicted SEED Role

"Purine nucleoside phosphorylase (EC 2.4.2.1)" in subsystem Adenosyl nucleosidases or Deoxyribose and Deoxynucleoside Catabolism or Purine conversions (EC 2.4.2.1)

MetaCyc Pathways

• superpathway of purine nucleotide salvage (13/14 steps found)
• superpathway of purine deoxyribonucleosides degradation (7/7 steps found)
• purine ribonucleosides degradation (6/6 steps found)
• superpathway of pyrimidine deoxyribonucleosides degradation (6/6 steps found)
• adenine and adenosine salvage III (4/4 steps found)
• purine deoxyribonucleosides degradation I (4/4 steps found)
• purine nucleotides degradation II (aerobic) (9/11 steps found)
• ureide biosynthesis (6/7 steps found)
• adenine and adenosine salvage V (3/3 steps found)
• purine deoxyribonucleosides degradation II (3/3 steps found)
• pyrimidine deoxyribonucleosides degradation (3/3 steps found)
• superpathway of guanine and guanosine salvage (3/3 steps found)
• adenine and adenosine salvage I (2/2 steps found)
• guanine and guanosine salvage I (2/2 steps found)
• xanthine and xanthosine salvage (2/2 steps found)
• guanosine nucleotides degradation III (3/4 steps found)
• inosine 5'-phosphate degradation (3/4 steps found)
• adenosine nucleotides degradation II (3/5 steps found)
• nucleoside and nucleotide degradation (archaea) (4/10 steps found)
• fluoroacetate and fluorothreonine biosynthesis (1/6 steps found)
• nucleoside and nucleotide degradation (halobacteria) (1/6 steps found)
• arsenic detoxification (mammals) (8/17 steps found)
• salinosporamide A biosynthesis (3/15 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.1

Use Curated BLAST to search for 2.4.2.1 or 2.4.2.15

Protein Sequence (240 amino acids)

>EX28DRAFT_3593 purine-nucleoside phosphorylase, family 1 (deoD) (Enterobacter asburiae PDN3)
MATPHINAEMGDFADVVLMPGDPLRAKHIAETFLEEVREVNNVRGMLGFTGTYKGRKISV
MGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVRMDVKLRDIVIGMGACTDSKVNRMR
FKDHDFAAIADFGMVRDAVDAAKALGVEARVGNIFSADLFYAPDGGEMFDVMEKYGILGV
EMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAERQTTFNDMIKIALESVLLGDKE