Protein Info for EX28DRAFT_3501 in Enterobacter asburiae PDN3

Annotation: Predicted metal-dependent hydrolase with the TIM-barrel fold

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 623 PF07969: Amidohydro_3" amino acids 54 to 538 (485 residues), 451.1 bits, see alignment E=7.7e-139 PF01979: Amidohydro_1" amino acids 413 to 537 (125 residues), 26.8 bits, see alignment E=3.1e-10

Best Hits

KEGG orthology group: K07047, (no description) (inferred from 99% identity to enc:ECL_00712)

Predicted SEED Role

"Exoenzymes regulatory protein AepA precursor"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (623 amino acids)

>EX28DRAFT_3501 Predicted metal-dependent hydrolase with the TIM-barrel fold (Enterobacter asburiae PDN3)
MVTLGKADLILVNGQFHTVDRENPVAEAVAVRDGKFLEVGTVAEVMQHHCEGTKVVDLKG
HTAIPGLNDSHLHLIRGGLNYNLELRWEGVPSLADALRMLKEQALRTPSPQWVRVVGGWT
EFQFAERRMPTLDEINDAAPDTPVFILHLYDRALLNRAALKVVGYTKDTPNPPGGEIQRD
ANGNPTGMLIARPNAMILYATLAKGPKLPLEQQVNSTRQFMRELNRLGLTSAIDAGGGFQ
NYPEDYEVIAELHEKKQMTIRIAYNLFTQRPGHELEDFEKWTDMLTPGQGSDFFRHNGAG
EMLVFSAADFEDFLEPRPDLAPGMEDELERVVRHLVEHRWPFRLHATYNESISRMLDVFE
KVNRDIPFNGLHWFFDHAETVTQANIDRIKALGGGIAVQHRMAFQGEYFAERYGLEATRH
TPPVAKMLETGVPVGLGTDATRVASYNPWTALYWLVSGRTVGGMQMYDHSARLDRDTALM
LWTQGSAWFSSEQNQKGQIKVGQLADLAVLSKDYFRVPEEEIKGIESVLTVVNGDIVYAA
GSFGPLAPPAIPVLPEWSPVVKVPGHYRSAPPQAARVGMSAVHHCSGPCGVHSHQHDFAR
TSEMPVSDDNAFWGALGCSCFAF