Protein Info for EX28DRAFT_3491 in Enterobacter asburiae PDN3

Annotation: tryptophanyl-tRNA synthetase (EC 6.1.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 333 PF00579: tRNA-synt_1b" amino acids 4 to 283 (280 residues), 170.8 bits, see alignment E=2.1e-54 TIGR00233: tryptophan--tRNA ligase" amino acids 4 to 329 (326 residues), 212.6 bits, see alignment E=4.5e-67

Best Hits

Swiss-Prot: 55% identical to SYW_CLOLO: Tryptophan--tRNA ligase (trpS) from Clostridium longisporum

KEGG orthology group: K01867, tryptophanyl-tRNA synthetase [EC: 6.1.1.2] (inferred from 98% identity to enc:ECL_00704)

Predicted SEED Role

"Tryptophanyl-tRNA synthetase (EC 6.1.1.2)" (EC 6.1.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.2

Use Curated BLAST to search for 6.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (333 amino acids)

>EX28DRAFT_3491 tryptophanyl-tRNA synthetase (EC 6.1.1.2) (Enterobacter asburiae PDN3)
MNAPTILTGDRPTGPLHLGHFVGSLRQRVALQQTHSQFVLIADLQGLTDNGSNPQKIRDN
IPEVLADYLAAGIDPNLTTICLQSALPALAELTMLYMNIVTVARVERNPTVKNEIAQKGF
ARSLPVGFMAYPISQAADITAFKAECVPVGDDQLPMIEQTNEIVHKMNSLLPAPVLRHCK
AMLSDTSRLPGIDGSAKMSKSLGNTLHLSASEETIHRAVSAMYTDPNHLKVSDPGQIEGN
VVFTYLDAFHPDKEKVAAMKAHYQLGGLGDRVCKNELEGCLQELIAPMRERRAMYMRDRG
ELMAMLKRGTERAQGVTQATLKELKVRLGVPVF