Protein Info for EX28DRAFT_3454 in Enterobacter asburiae PDN3
Annotation: Dihydrodipicolinate synthase/N-acetylneuraminate lyase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 98% identical to YAGE_ECOLI: Putative 2-dehydro-3-deoxy-D-gluconate aldolase YagE (yagE) from Escherichia coli (strain K12)
KEGG orthology group: K01714, dihydrodipicolinate synthase [EC: 4.2.1.52] (inferred from 98% identity to eco:b0268)MetaCyc: 98% identical to CP4-6 prophage; putative 2-dehydro-3-deoxygluconate aldolase (Escherichia coli K-12 substr. MG1655)
DHDOGALDOL-RXN [EC: 4.1.2.51]; 2-dehydro-3-deoxy-D-pentonate aldolase. [EC: 4.1.2.51, 4.1.2.28]
Predicted SEED Role
"4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7)" (EC 4.3.3.7)
MetaCyc Pathways
- aspartate superpathway (24/25 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (18/18 steps found)
- L-lysine biosynthesis I (9/9 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
- Entner-Doudoroff pathway II (non-phosphorylative) (8/9 steps found)
- L-lysine biosynthesis III (6/7 steps found)
- L-lysine biosynthesis VI (6/7 steps found)
- D-xylose degradation to ethylene glycol (engineered) (3/4 steps found)
- dipicolinate biosynthesis (3/4 steps found)
- L-lysine biosynthesis II (6/9 steps found)
- D-xylose degradation IV (4/7 steps found)
- superpathway of pentose and pentitol degradation (13/42 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Lysine biosynthesis
- Pentose and glucuronate interconversions
Isozymes
Compare fitness of predicted isozymes for: 4.2.1.52, 4.3.3.7
Use Curated BLAST to search for 4.1.2.28 or 4.1.2.51 or 4.2.1.52 or 4.3.3.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (302 amino acids)
>EX28DRAFT_3454 Dihydrodipicolinate synthase/N-acetylneuraminate lyase (Enterobacter asburiae PDN3) MPQSALFTGIIPPVSTIFTADGQLDKPGTAALIDDLIKAGVDGLFFLGSGGEFSQLSAEE RKTIARFAIDHVDRRVPVLIGTGGTNARETIELSQHAQQAGADGIVVINPYYWKVSEANL IRYFEQVADSVTLPVMLYNFPALTGQDLTPALVKTLADSRSNIIGIKDTIDSVAHLRSMI HTVKGAHPRFTVLCGYDDHLFNTLLLGGDGAISASGNFAPQVSVNLLKAWRDGDVAKAAE YHQTLLQIPQMYQLDTPFVNVIKEAIVLCGRPVSTHVLPPASPLDEPRRAQLKTLLQQLK LC