Protein Info for EX28DRAFT_3443 in Enterobacter asburiae PDN3

Annotation: Glycosyl hydrolase family 20, catalytic domain/Glycosyl hydrolase family 20, domain 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 794 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF02838: Glyco_hydro_20b" amino acids 26 to 155 (130 residues), 61.1 bits, see alignment E=2.6e-20 PF14845: Glycohydro_20b2" amino acids 28 to 138 (111 residues), 42 bits, see alignment E=2.3e-14 PF00728: Glyco_hydro_20" amino acids 159 to 418 (260 residues), 292.4 bits, see alignment E=8.7e-91 amino acids 544 to 584 (41 residues), 32.9 bits, see alignment 7.2e-12

Best Hits

KEGG orthology group: K12373, hexosaminidase [EC: 3.2.1.52] (inferred from 95% identity to enc:ECL_00661)

Predicted SEED Role

"Beta-hexosaminidase (EC 3.2.1.52)" in subsystem Chitin and N-acetylglucosamine utilization or N-Acetyl-Galactosamine and Galactosamine Utilization (EC 3.2.1.52)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.52

Use Curated BLAST to search for 3.2.1.52

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (794 amino acids)

>EX28DRAFT_3443 Glycosyl hydrolase family 20, catalytic domain/Glycosyl hydrolase family 20, domain 2 (Enterobacter asburiae PDN3)
MLRYSLLTAGLMLGASAFAAPAGDLPLMPWPAKVERPTTQGALVLNDKISVSVSGDDLGD
AVNRLRQRMALQTGWTLQPQAEKPDKPTIRIAVAKKVKPQPLPDSDESYRLTVDANGVDI
SANTRFGALRAMETLLQLMQNGAENTSLPWVTIEDSPRFSWRGLLLDSARHFIPLPDIKR
QIDGMAAAKLNVLHWHLTDDQGWRFSSKRYPKLTQLASDGLFYTPEQMREVVRYAAERGI
RVVPEIDMPGHASAIAVAYPELMSAPGPYAMERHWGVLKPVLDPTKEATYAFADAMVSEL
AAIFPDPYLHIGGDEVDDSQWKANAAIQNFMRDNRLADSHALQAYFNRKLETILEKHHRQ
MVGWDEIYHPDLPKSILIQSWQGQDALGQVAQNGYKGILSTGFYLDQPQSTAYHYRNEIV
PQGLNGVDVIADTDSVQSWAFSMPRLKGKPVEGSFTLVKGDAGWRGFIDFAGKSRRAVQD
IEWRDDSQVTFTVDTWMGETRPVVSVDNDKLTGYFLVGNTRYPISGTRLDAVPKGIPPVV
PDVANQANLLGGEAALWAENVVAPVLDIRLWPRTFAVAERLWSAQDVNDVDNMYTRLQAM
DSWSTVSVGLQQHTQQQVQFTRLAGNADTLPLQILAQAVEPAQYYTRQHLKFQAGNYHQF
EPLNRFADALNAESATVRQMNKWADRLVSDAEDAESADALRHVFNRWQSNTSDALALSEN
SYQLKAMKPVIQEVDKLASIGLRLTDLVARQGTLDDKEIASIQSELDNAAKVQDEVVIAA
VYPLETLLRATRNQ