Protein Info for EX28DRAFT_3437 in Enterobacter asburiae PDN3
Annotation: ribonucleoside-triphosphate reductase class III catalytic subunit (EC 1.17.4.2)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 95% identical to NRDD_SALTY: Anaerobic ribonucleoside-triphosphate reductase (nrdD) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
KEGG orthology group: K00527, ribonucleoside-triphosphate reductase [EC: 1.17.4.2] (inferred from 99% identity to enc:ECL_00656)MetaCyc: 94% identical to anaerobic ribonucleoside-triphosphate reductase (Escherichia coli K-12 substr. MG1655)
RIBONUCLEOSIDE-TRIP-REDUCT-RXN [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]
Predicted SEED Role
"Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2)" in subsystem Ribonucleotide reduction (EC 1.17.4.2)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
- superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
- superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
- superpathway of guanosine nucleotides de novo biosynthesis II (8/8 steps found)
- pyrimidine deoxyribonucleotides de novo biosynthesis II (7/7 steps found)
- superpathway of adenosine nucleotides de novo biosynthesis II (7/7 steps found)
- adenosine deoxyribonucleotides de novo biosynthesis II (4/4 steps found)
- guanosine deoxyribonucleotides de novo biosynthesis II (4/4 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.1.98.6 or 1.17.4.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (712 amino acids)
>EX28DRAFT_3437 ribonucleoside-triphosphate reductase class III catalytic subunit (EC 1.17.4.2) (Enterobacter asburiae PDN3) MTPHVMKRDGCKVPFKSERIQEAILRAAKAAGVDDADYCATVAEVVSSQMNERSQVDINE IQTAVENQLMAGPYKQLARAYIEYRHDRDVQREKRGRLNQEIRGLVEQTNSALLNENANK DSKVIPTQRDLLAGIVAKHYARQHLLPRDVVSAHERGEIHYHDLDYSPFFPMFNCMLIDL KGMLTHGFKMGNAEIEPPKSISTATAVTAQIIAQVASHIYGGTTINRIDEVLAPFVTASF NKHRKTAEEWQIPDADGYAHSRTEKECYDAFQSLEYEVNTLHTANGQTPFVTFGFGLGTS WESRLIQQSILRNRISGLGKNRKTAVFPKLVFAIRDGLNHKFGDPNYDIKQLALECASKR MYPDILNYDQVVKVTGSFKTPMGCRSFLGVYEDENGEQIHDGRNNLGVISLNLPRIALEA KGNEDAFWTLLDERLQLARKALMTRIARLEGVKARVAPILYMEGACGVRLKADDDVSEIF KNGRASISLGYIGIHETINALAGDTHMYDSEALREKGIAIVQRLRDAVDQWKEETGYGFS LYSTPSENLCDRFCRLDTAEFGIVEGVTDKGYYTNSFHLDVEKKVNPYDKIDFEAAYPPI ASGGFICYGEYPNIQHNLKALEDVWDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGF TCPKCGNHDAARVSVTRRVCGYLGSPDARPFNAGKQEEVKRRVKHLGNGQIG