Protein Info for EX28DRAFT_3430 in Enterobacter asburiae PDN3

Annotation: uncharacterized pyridoxal phosphate-dependent enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01437: uncharacterized pyridoxal phosphate-dependent enzyme
  • amino acids 4 to 365 (362 residues), 606.2 bits, E=9.3e-187
• PF03841: SelA
  • amino acids 14 to 336 (323 residues), 52.2 bits, E=5e-18

Best Hits

KEGG orthology group: K01042, L-seryl-tRNA(Ser) seleniumtransferase [EC: 2.9.1.1] (inferred from 98% identity to enc:ECL_00648)

Predicted SEED Role

"D-Glucosaminate-6-phosphate ammonia-lyase (EC 4.3.1.-)" (EC 4.3.1.-)

MetaCyc Pathways

• L-selenocysteine biosynthesis I (bacteria) (3/3 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Selenoamino acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.9.1.1

Use Curated BLAST to search for 2.9.1.1 or 4.3.1.-

Protein Sequence (372 amino acids)

>EX28DRAFT_3430 uncharacterized pyridoxal phosphate-dependent enzyme (Enterobacter asburiae PDN3)
MPSIYEKYNLKQVINTSGRMTALGVSTPRPEVVQAAMDGMNHYFEMKDLINKTGEYIAKL
LDVEGATVVSCASAGIAQSVAAVLVKDSDWLLENLHVTPIENNEIVLPKGHNVNFGAPVG
TMVALGGGKLVEAGYANECSADQLAAAITPRTAAILYIKSHHCVQKSMLSVEQAAVVARK
HDLPLIVDAAAEEDLHAYYRSGADLVIYSGAKAIEGPTSGLVIGKTQYVEWVKRQTAGIG
RAMKVGKEGILGLTCAIEHYLTATKESGAEMVAKMTPFIDALNTLNGVTARVVWDSAGRD
IARTEIKFDEAATGVGTGDLVAKLKQGEYAIYFRGYKANEGIIEADVRSVNADQLNIVYR
RISEVLGQEKKA