Protein Info for EX28DRAFT_3383 in Enterobacter asburiae PDN3

Annotation: Alpha/beta hydrolase family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 240 transmembrane" amino acids 43 to 47 (5 residues), see Phobius details PF01738: DLH" amino acids 18 to 214 (197 residues), 24.1 bits, see alignment E=8.5e-09 PF03403: PAF-AH_p_II" amino acids 24 to 71 (48 residues), 26 bits, see alignment 1.2e-09 PF12146: Hydrolase_4" amino acids 25 to 143 (119 residues), 39.2 bits, see alignment E=1.7e-13 PF00561: Abhydrolase_1" amino acids 28 to 168 (141 residues), 35.2 bits, see alignment E=3.7e-12 PF12697: Abhydrolase_6" amino acids 30 to 188 (159 residues), 31.1 bits, see alignment E=1.4e-10 PF00326: Peptidase_S9" amino acids 50 to 236 (187 residues), 48.5 bits, see alignment E=2.8e-16

Best Hits

Swiss-Prot: 61% identical to YJFP_ECOLI: Esterase YjfP (yjfP) from Escherichia coli (strain K12)

KEGG orthology group: K06889, (no description) (inferred from 88% identity to enc:ECL_00584)

MetaCyc: 61% identical to carboxylesterase (Escherichia coli K-12 substr. MG1655)
Carboxylesterase. [EC: 3.1.1.1]

Predicted SEED Role

"YjfP protein"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.1

Use Curated BLAST to search for 3.1.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (240 amino acids)

>EX28DRAFT_3383 Alpha/beta hydrolase family (Enterobacter asburiae PDN3)
MIEIEIRHLGDHEILHAMPAGKRDKPLPVVVFYHGFTSSKLVYSYFAVALAQAGFRVVMP
DAPDHGARFTGNEQARLGQFWQILHGSLTEFAGLRDALYEAGLVADNRLAVAGASMGGMT
ALGIMTHHPEVKSVACLMGSGYFTSLAKTLFPPQDLTEMTATLAEWEVTRALPRVADRPL
LLWHGDADDVVPPEGTFRLQQALMNEGLDGNLTCLWEPGVRHRITPTALDATVAFFRQHL