Protein Info for EX28DRAFT_3374 in Enterobacter asburiae PDN3

Annotation: Adenylosuccinate synthetase (EC 6.3.4.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00709: Adenylsucc_synt
  • amino acids 20 to 438 (419 residues), 607 bits, E=8.4e-187
• TIGR00184: adenylosuccinate synthase
  • amino acids 20 to 444 (425 residues), 680.2 bits, E=5.6e-209

Best Hits

Swiss-Prot: 98% identical to PURA_ENT38: Adenylosuccinate synthetase (purA) from Enterobacter sp. (strain 638)

KEGG orthology group: K01939, adenylosuccinate synthase [EC: 6.3.4.4] (inferred from 100% identity to enc:ECL_00575)

MetaCyc: 95% identical to adenylosuccinate synthetase (Escherichia coli K-12 substr. MG1655)
Adenylosuccinate synthase. [EC: 6.3.4.4]

Predicted SEED Role

"Adenylosuccinate synthetase (EC 6.3.4.4)" in subsystem CBSS-262719.3.peg.410 or Purine conversions (EC 6.3.4.4)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (7/7 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
• adenosine ribonucleotides de novo biosynthesis (3/3 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Biosynthesis of alkaloids derived from histidine and purine
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.4

Protein Sequence (446 amino acids)

>EX28DRAFT_3374 Adenylosuccinate synthetase (EC 6.3.4.4) (Enterobacter asburiae PDN3)
MVESIFKQTVILKKMGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTLVI
NGEKTVLHLIPSGILRDNVTSIIGNGVVLSPAALMKEMKGLEDRGIPVRERLLLSEACPL
ILDYHVALDVAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKATFAEKLKEVM
EYHNFQLVNFYKADAVDYQKVLDDVMAIADILTGMVVDVSDLLDQARKRGDFVMFEGAQG
TLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGIIKAYSTRVGAGPFPTELFDE
TGEFLCKQGNEFGATTGRRRRTGWLDAVAVRRAVQINSLSGFCLTKLDVLDGLKEVKICV
GYRMPDGREVTTTPLAADDWEGIEPIYETMPGWSETTFGVKERSGLPQAALDYIKRIEEL
TEVPIDIISTGPDRTETMILRDPFDA