Protein Info for EX28DRAFT_3364 in Enterobacter asburiae PDN3

Annotation: yjeF C-terminal region, hydroxyethylthiazole kinase-related/yjeF N-terminal region

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 502 TIGR00197: YjeF family N-terminal domain" amino acids 13 to 215 (203 residues), 202.3 bits, see alignment E=6.6e-64 PF03853: YjeF_N" amino acids 35 to 194 (160 residues), 156.1 bits, see alignment E=8.5e-50 TIGR00196: YjeF family C-terminal domain" amino acids 230 to 493 (264 residues), 307.5 bits, see alignment E=8.4e-96 PF01256: Carb_kinase" amino acids 253 to 489 (237 residues), 263.5 bits, see alignment E=1.9e-82

Best Hits

Swiss-Prot: 82% identical to NNR_ECOLI: Bifunctional NAD(P)H-hydrate repair enzyme Nnr (nnr) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 92% identity to enc:ECL_00565)

MetaCyc: 82% identical to NAD(P)HX epimerase / NAD(P)HX dehydratase (Escherichia coli K-12 substr. MG1655)
RXN-12752 [EC: 5.1.99.6]; 5.1.99.6 [EC: 5.1.99.6]; RXN-13141 [EC: 5.1.99.6, 4.2.1.136]; 4.2.1.136 [EC: 5.1.99.6, 4.2.1.136]

Predicted SEED Role

"NAD(P)HX epimerase / NAD(P)HX dehydratase"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.136 or 5.1.99.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (502 amino acids)

>EX28DRAFT_3364 yjeF C-terminal region, hydroxyethylthiazole kinase-related/yjeF N-terminal region (Enterobacter asburiae PDN3)
MKKNPASIPHSIWHADDLRRAEKEAADSLGITLYELMLRAGEAAFTVARSAYPQAAHWLI
LCGHGNNGGDGYVVARLAVAAGIRVTLLALESDKPLPEEASAAREAWLNAGGVIHAPDIV
WPEEVDLIVDGLLGTGLRSAPRENIAALIEHANRHSAPIVALDIPSGLIAQTGATPGAVI
EAAHTVTFIALKPGLLTGKARDVVGVLHHNALGLESWLAGQEMRIARFDASQLAAWLPPR
RPTSHKGDHGRLVIIGGDHGTAGAIRMTGEAALRSGAGLVRVLTRIENIAPIVTARPELM
VHELTPQALEESLEWADVVVIGPGLGQQAWGKQALQKVENFRKPMLWDADALNLLAINPD
KRHNRILTPHPGEAARLLNCSVAEIESDRLLSAQRLVKRYGGVAVLKGAGTVVASDETQG
IIDAGNAGMASGGMGDVLSGIIGALLGQKLPLYDAACAGCVAHGVAADKLAARYGTRGML
ATDLFCTLRRVVNPDVIDVEND