Protein Info for EX28DRAFT_3359 in Enterobacter asburiae PDN3

Annotation: ribosome small subunit-dependent GTPase A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00157: ribosome small subunit-dependent GTPase A
  • amino acids 73 to 334 (262 residues), 326.9 bits, E=4.2e-102
• PF03193: RsgA_GTPase
  • amino acids 100 to 276 (177 residues), 200.9 bits, E=2.1e-63

Best Hits

Swiss-Prot: 92% identical to RSGA_ECO55: Small ribosomal subunit biogenesis GTPase RsgA (rsgA) from Escherichia coli (strain 55989 / EAEC)

KEGG orthology group: K06949, ribosome biogenesis GTPase [EC: 3.6.1.-] (inferred from 97% identity to enc:ECL_00562)

MetaCyc: 91% identical to ribosome small subunit-dependent GTPase A (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Ribosome small subunit-stimulated GTPase EngC" in subsystem Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Folate biosynthesis
• Lipopolysaccharide biosynthesis
• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.1.15

Use Curated BLAST to search for 3.6.1.- or 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Protein Sequence (350 amino acids)

>EX28DRAFT_3359 ribosome small subunit-dependent GTPase A (Enterobacter asburiae PDN3)
MSKNKLSKGQQRRVNANHQRRLKTTSEKPDYDDNLFGEATEGVVISRFGMHADVESADGD
IHRCNIRRTIRSLVTGDRVVWRPGKEAAEGVTVKGIVEAVHERTSVLTRPDFYDGVKPIA
ANINQIVIVSAILPELSLNIIDRYLVACETLQVEPIIVLNKIDLLDDDGMAFVNEQMDIY
RKIGYRVLMVSSHTKDGLKPLEEALTDRISIFAGQSGVGKSSLLNNLLGLQQEILTNDVS
NVSGLGQHTTTASRLYHFPHGGDVIDSPGVREFGLWHLEPEQIFNGFVEFHDYLGACKYR
DCKHDNDPGCAIREAVEKGEIAETRFENYHRILESMEDVKTRKNFSDSDN