Protein Info for EX28DRAFT_3291 in Enterobacter asburiae PDN3

Annotation: Predicted membrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 655 transmembrane" amino acids 13 to 32 (20 residues), see Phobius details amino acids 38 to 56 (19 residues), see Phobius details amino acids 68 to 88 (21 residues), see Phobius details amino acids 94 to 113 (20 residues), see Phobius details amino acids 119 to 138 (20 residues), see Phobius details amino acids 151 to 168 (18 residues), see Phobius details amino acids 356 to 374 (19 residues), see Phobius details amino acids 380 to 397 (18 residues), see Phobius details amino acids 407 to 426 (20 residues), see Phobius details amino acids 432 to 449 (18 residues), see Phobius details amino acids 456 to 476 (21 residues), see Phobius details amino acids 486 to 503 (18 residues), see Phobius details PF04632: FUSC" amino acids 11 to 653 (643 residues), 497.4 bits, see alignment E=8.3e-153 PF13515: FUSC_2" amino acids 23 to 162 (140 residues), 47.4 bits, see alignment E=2.2e-16

Best Hits

Swiss-Prot: 96% identical to AAEB_ENTCC: p-hydroxybenzoic acid efflux pump subunit AaeB (aaeB) from Enterobacter cloacae subsp. cloacae (strain ATCC 13047 / DSM 30054 / NBRC 13535 / NCDC 279-56)

KEGG orthology group: None (inferred from 96% identity to enc:ECL_04623)

MetaCyc: 86% identical to aromatic carboxylic acid efflux pump subunit AaeB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-233

Predicted SEED Role

"FUSARIC ACID RESISTANCE PROTEIN FUSB / FUSARIC ACID RESISTANCE PROTEIN FUSC"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (655 amino acids)

>EX28DRAFT_3291 Predicted membrane protein (Enterobacter asburiae PDN3)
MGIFSIASQHIRFAVKLACAIVLALFVGFHFQLETPRWAVLTAAIVAAGPAFAAGGEPYS
GAIRYRGMLRIIGTFIGCFAALTIIILMIRTPLLMLMVCCIWAGFCTWISSLVKVENSYA
WGLAGYTALIIIITIQSEPLLAPQFAVERCSEIVIGIVSAIVADLLFSPRSIKQEVDREL
DALIVAQYQLMQLCIKHGDSEEVDKAWSGLVRRTQALEGMRSNLNMESSRWARANRRLKA
LNTVSLTLITQACETYLIQNTRPEAVTDTFRELFAEPVETVQDVHKQLKRMRRVIAWTGE
RDTPVTIYTWVGAATRYLLLKRGVIGNTKISAAEEEVLQGEVVIKAESAERHHAMVNFWR
TTLACMLGTLFWLWTGWTSGSGAMVMIAVVTALAMRLPNPRMVAVDFLYGTIAALPIGAL
YFLVIIPSTQQSMLLLCISLAVMAFFIGIEVQKRRLGSLGALASTINIIVLDNPMTFHFS
QFLDSALGQLVGCFLAMMVILLVRDNSQARTGRVLLNQFVSAAVSSLTTNTARRKENHLP
ALYQQLFLLLNKFPGDIGKFRLALTMIIAHQRLRNAPVPINDDLSAWHRQLRRTADHVLS
ASSDDKRRRYFTQLLEELDIYQEKLKHWEAPPQVTEPVGRLVFMLHRYQNALTDN