Protein Info for EX28DRAFT_3285 in Enterobacter asburiae PDN3

Annotation: malate dehydrogenase (NAD) (EC 1.1.1.37)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00056: Ldh_1_N
  • amino acids 1 to 145 (145 residues), 155.9 bits, E=7.1e-50
• TIGR01772: malate dehydrogenase, NAD-dependent
  • amino acids 2 to 311 (310 residues), 464.1 bits, E=1.2e-143
• PF02866: Ldh_1_C
  • amino acids 147 to 310 (164 residues), 173 bits, E=6.3e-55

Best Hits

Swiss-Prot: 96% identical to MDH_ENT38: Malate dehydrogenase (mdh) from Enterobacter sp. (strain 638)

KEGG orthology group: K00024, malate dehydrogenase [EC: 1.1.1.37] (inferred from 98% identity to enc:ECL_04617)

MetaCyc: 96% identical to malate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Malate dehydrogenase. [EC: 1.1.1.37, 1.1.1.38]

Predicted SEED Role

"Malate dehydrogenase (EC 1.1.1.37)" in subsystem Serine-glyoxylate cycle or TCA Cycle (EC 1.1.1.37)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
• mixed acid fermentation (16/16 steps found)
• gluconeogenesis I (13/13 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• glyoxylate cycle (6/6 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle III (animals) (8/10 steps found)
• TCA cycle VIII (Chlamydia) (5/6 steps found)
• malate/L-aspartate shuttle pathway (2/2 steps found)
• TCA cycle II (plants and fungi) (7/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (7/9 steps found)
• gluconeogenesis III (9/12 steps found)
• L-malate degradation II (1/1 steps found)
• chitin deacetylation (3/4 steps found)
• C4 photosynthetic carbon assimilation cycle, NAD-ME type (8/11 steps found)
• reductive TCA cycle I (8/11 steps found)
• anaerobic energy metabolism (invertebrates, cytosol) (5/7 steps found)
• incomplete reductive TCA cycle (5/7 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (6/9 steps found)
• reductive TCA cycle II (8/12 steps found)
• L-carnitine degradation II (1/3 steps found)
• methylgallate degradation (3/6 steps found)
• formaldehyde assimilation I (serine pathway) (8/13 steps found)
• protocatechuate degradation I (meta-cleavage pathway) (4/8 steps found)
• pyruvate fermentation to propanoate I (3/7 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (5/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (10/17 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)
• superpathway of vanillin and vanillate degradation (4/10 steps found)
• syringate degradation (4/12 steps found)
• L-glutamate degradation VIII (to propanoate) (3/11 steps found)
• methylaspartate cycle (8/19 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (23/56 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Carbon fixation in photosynthetic organisms
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.38

Use Curated BLAST to search for 1.1.1.37 or 1.1.1.38

Protein Sequence (312 amino acids)

>EX28DRAFT_3285 malate dehydrogenase (NAD) (EC 1.1.1.37) (Enterobacter asburiae PDN3)
MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAVDLSHIPTAVKIKGFSG
EDARPALQGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLVQQIAEICPKACIGIITNP
VNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAELKGKQPTEVEVPVIGGHSGV
TILPLLSQIPGVSFTEQEVADLTKRIQNAGTEVVEAKAGGGSATLSMGQAAARFGLSLVR
ALQGEKGVVECAYVEGDGEHARFFSQPLLLGKNGIEERKSIGTLSAFEQHAMEGMLDTLK
KDITLGEEFVNK