Protein Info for EX28DRAFT_3275 in Enterobacter asburiae PDN3

Annotation: Glutamate synthase domain 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00310: GATase_2
  • amino acids 12 to 425 (414 residues), 593 bits, E=5.9e-182
• PF04898: Glu_syn_central
  • amino acids 458 to 737 (280 residues), 356.5 bits, E=2.1e-110
• PF01645: Glu_synthase
  • amino acids 793 to 1156 (364 residues), 524.2 bits, E=4.1e-161
• PF01493: GXGXG
  • amino acids 1236 to 1419 (184 residues), 255.6 bits, E=4.9e-80

Best Hits

Swiss-Prot: 94% identical to GLTB_ECOLI: Glutamate synthase [NADPH] large chain (gltB) from Escherichia coli (strain K12)

MetaCyc: 94% identical to glutamate synthase subunit GltB (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Glutamate synthase [NADPH] large chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)

MetaCyc Pathways

• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamine degradation II (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.13

Use Curated BLAST to search for 1.4.1.13

Protein Sequence (1486 amino acids)

>EX28DRAFT_3275 Glutamate synthase domain 2 (Enterobacter asburiae PDN3)
MLYDKSLEKDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGAILADGKTGDGCGLLLQ
KPDRFFRIVAEERGWRLAKNYAVGMLFLNQDPEKAAASRRIVEEELQRETLSIVGWRNVP
TNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIARRRIEKRLQDDKEFYVCSLS
NLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRFSTNTVPRWPLAQPFRYLAHNGE
INTITGNRQWARARTYKFQTPLIPDLHDAAPFVNETGSDSSSMDNMLELLLAGGMDIVRA
MRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWDGPAGIVMSDGRFAACNLDRNGLRPAR
YVITKDKLITCASEVGIWDYQPDEVVEKGRVGPGELMVIDTRGGRILHSAETDHDLKSRH
PYKEWMEKNVRRLVPFEDLSDEEVGRRELDDDMLASFQKQFNYSAEELDSVIRVLGENGQ
EAVGSMGDDTPFAVLSSQPRIIYDYFRQQFAQVTNPPIDPLREAHVMSLATSIGREMNVF
CEAEGQAHRLTFKSPILLYSDFKQLTTMKEEHYRADTLDITFDVTEASLEETVNTLCDKA
EQMVRNGTVLLVLSDRNIAKNRLPVPAPMAVGAIQTRLVDKSLRCDANIIVETASARDPH
HFAVLLGFGATAIYPYLAYETLARLVDTRAIDKDYRAVMLNYRNGINKGLYKIMSKMGIS
TIASYRCSKLFEAVGLHDEVANLCFQGVVSRIGGAGFADFQQDLINLSKRAWLARKPLDQ
GGLLKYVHGGEYHAYNPDVVRTLQQAVQSGEYSDYQQYAELVNNRPAATLRDLIALNPGD
EAVSIDEVEPASELFKRFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYG
TNKVSRIKQVASGRFGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSV
PGVTLISPPPHHDIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADL
ITIAGYDGGTGASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGLDII
KAAILGAESFGFGTGPMVALGCKYLRICHLNNCATGVATQDEKLRKNHYHGLPFKVTNYF
EFIARETRELMAQLGVKRLVDLIGRTDLLKELEGFTAKQQKLELSKLLETAQPHPGKAVY
CTENNPPFDNGVLNAQLLQQAKPYVDDKQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQG
LAADPITAHFSGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLLAVRPPVGSAFRSH
EASIIGNTCLYGATGGRLFAAGRAGERFAVRNSGAITVVEGIGDNGCEYMTGGIVCVLGK
TGVNFGAGMTGGFAYVLDEDGEFRKRVNPELVEVLDVDTLAIHEEHLRGLITEHVQHTGS
SRGEEILANWPAFSAKFALVKPKSSDVKALLGHRSRSAAELRVQAQ