Protein Info for EX28DRAFT_3211 in Enterobacter asburiae PDN3

Annotation: Superfamily II DNA and RNA helicases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 631 PF00270: DEAD" amino acids 30 to 196 (167 residues), 172.3 bits, see alignment E=1.9e-54 PF04851: ResIII" amino acids 45 to 158 (114 residues), 22.6 bits, see alignment E=2.2e-08 PF00271: Helicase_C" amino acids 232 to 340 (109 residues), 103.6 bits, see alignment E=1.9e-33 PF03880: DbpA" amino acids 499 to 568 (70 residues), 71.8 bits, see alignment E=9.6e-24 PF12343: DeaD_C" amino acids 580 to 630 (51 residues), 24.4 bits, see alignment 7.7e-09

Best Hits

Swiss-Prot: 91% identical to DEAD_ECOLI: ATP-dependent RNA helicase DeaD (deaD) from Escherichia coli (strain K12)

KEGG orthology group: K05592, ATP-dependent RNA helicase DeaD [EC: 3.6.4.13] (inferred from 91% identity to eco:b3162)

MetaCyc: 91% identical to ATP-dependent RNA helicase DeaD (Escherichia coli K-12 substr. MG1655)
5.6.2.e [EC: 5.6.2.e]

Predicted SEED Role

"DEAD-box ATP-dependent RNA helicase CshA (EC 3.6.4.13)" (EC 3.6.4.13)

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13 or 5.6.2.e

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (631 amino acids)

>EX28DRAFT_3211 Superfamily II DNA and RNA helicases (Enterobacter asburiae PDN3)
MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLSGRDVLGMAQTGSGKTAAF
SLPLLNNIDPDLRAPQILVLAPTRELAVQVAEAMTEFSKHMRGVNVVALYGGQRYDVQLR
ALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADEMLRMGFIEDVETIMAQIPDG
HQTALFSATMPEAIRRITKRFMKDPQEVRIQSSVTTRPDISQSYWSVYGMRKNEALVRFL
EAEDFDAAIIFVRTKNATLEVAEALERSGYNSAALNGDMNQALREQTLERLKDGRLDILI
ATDVAARGLDVERISLVVNYDIPMDSESYVHRIGRTGRAGRAGRALLFVENRERRLLRNI
ERTMKLTIPEADLPNADLLGKRRLEKFAAKVQQQLESSDLDQYRALLSQIQPVAEGEELD
METLAAALLKMAQGERSLIVPPDAPMRPKREFRDRDDRFERRGDRNDRNDRGPRGDRPER
GGEDRPRRERRDAGDMELYRIEVGRDDGVEVRHIVGAIANEGDISSRYIGNIKLFASHST
IELPKGMPGEVLQHFTRTRILNKPMNMQLMGDAQPRPDRGGERRGGGRSFGGERREGGRS
EGRGEPRRFSGERRENRGPRRDEGTSRRRDA