Protein Info for EX28DRAFT_3186 in Enterobacter asburiae PDN3

Annotation: D-tagatose-bisphosphate aldolase, class II, non-catalytic subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08013: GatZ_KbaZ-like
  • amino acids 4 to 423 (420 residues), 674.1 bits, E=3.5e-207
• TIGR02810: D-tagatose-bisphosphate aldolase, class II, non-catalytic subunit
  • amino acids 6 to 424 (419 residues), 702.5 bits, E=9.5e-216

Best Hits

Swiss-Prot: 89% identical to KBAZ_CITK8: D-tagatose-1,6-bisphosphate aldolase subunit KbaZ (kbaZ) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: K00917, tagatose 6-phosphate kinase [EC: 2.7.1.144] (inferred from 94% identity to enc:ECL_04519)

MetaCyc: 86% identical to D-tagatose-1,6-bisphosphate aldolase subunit KbaZ (Escherichia coli O157:H7)
Tagatose-bisphosphate aldolase. [EC: 4.1.2.40]

Predicted SEED Role

"Tagatose-6-phosphate kinase AgaZ (EC 2.7.1.144)" in subsystem N-Acetyl-Galactosamine and Galactosamine Utilization (EC 2.7.1.144)

MetaCyc Pathways

• superpathway of hexitol degradation (bacteria) (16/18 steps found)
• D-galactosamine and N-acetyl-D-galactosamine degradation (4/4 steps found)
• N-acetyl-D-galactosamine degradation (4/5 steps found)
• galactitol degradation (3/5 steps found)
• D-tagatose degradation (1/3 steps found)
• lactose degradation I (2/5 steps found)

KEGG Metabolic Maps

• Galactose metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.1.2.40

Use Curated BLAST to search for 2.7.1.144 or 4.1.2.40

Protein Sequence (431 amino acids)

>EX28DRAFT_3186 D-tagatose-bisphosphate aldolase, class II, non-catalytic subunit (Enterobacter asburiae PDN3)
MKHLTEMVEQHKRGNSNGIYAVCSAHPLVLEAAIRYAHSHQSPLLIEATSNQVDQFGGYT
GMTPADFYGFVCQLAESLGFPTSQLILGGDHLGPNRWQNLPAEQAMANADDLIKSYVSAG
FKKIHLDCSMSCEDDPVPLTDAIVAGRAARLAKIAEATCREQFGESDLVYVIGTEVPVPG
GAHETLTELAVTTPDAARATLEAHRHAFEKEGLSDIWPRIIGLVVQPGVEFDHAHVCDYQ
PQKAVALSKMVEAYDTLVFEAHSTDYQTPQALRQLVNDHFAILKVGPALTFALREALFSL
AAIEEELLPAKASSGLRHVLENVMLDRPEYWQSHYHGDGNARRLARGYSYSDRVRYYWPD
SQIDDAFARLVRNLADESVPLPLISQYLPLQYSKVREGALKSTPRELIIDHIQDILQQYH
AACEGVTTQDA