Protein Info for EX28DRAFT_3178 in Enterobacter asburiae PDN3
Annotation: Threonine dehydratase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 92% identical to TDCB_ECOL6: L-threonine dehydratase catabolic TdcB (tdcB) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
KEGG orthology group: K01754, threonine dehydratase [EC: 4.3.1.19] (inferred from 97% identity to enc:ECL_04511)MetaCyc: 92% identical to catabolic threonine dehydratase (Escherichia coli K-12 substr. MG1655)
Threonine ammonia-lyase. [EC: 4.3.1.19]; L-serine ammonia-lyase. [EC: 4.3.1.19, 4.3.1.17]
Predicted SEED Role
"Threonine dehydratase, catabolic (EC 4.3.1.19)" in subsystem Branched-Chain Amino Acid Biosynthesis or Glycine and Serine Utilization or Threonine anaerobic catabolism gene cluster or Threonine degradation (EC 4.3.1.19)
MetaCyc Pathways
- superpathway of branched chain amino acid biosynthesis (17/17 steps found)
- superpathway of L-isoleucine biosynthesis I (13/13 steps found)
- superpathway of L-threonine metabolism (16/18 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
- L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
- L-threonine degradation I (6/6 steps found)
- D-serine degradation (3/3 steps found)
- L-cysteine degradation II (3/3 steps found)
- L-serine degradation (3/3 steps found)
- glycine degradation (3/3 steps found)
- L-methionine biosynthesis II (5/6 steps found)
- L-methionine degradation II (2/3 steps found)
- L-tryptophan degradation II (via pyruvate) (2/3 steps found)
- L-threonine degradation V (1/2 steps found)
- glycine betaine degradation III (4/7 steps found)
- felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis (2/5 steps found)
- L-mimosine degradation (4/8 steps found)
- glycine betaine degradation I (4/8 steps found)
- glutathione-mediated detoxification I (3/8 steps found)
- purine nucleobases degradation II (anaerobic) (13/24 steps found)
- hypoglycin biosynthesis (4/14 steps found)
- cyclosporin A biosynthesis (2/15 steps found)
KEGG Metabolic Maps
- Cysteine metabolism
- Glycine, serine and threonine metabolism
- Valine, leucine and isoleucine biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 4.3.1.17, 4.3.1.19
Use Curated BLAST to search for 4.3.1.17 or 4.3.1.19
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (329 amino acids)
>EX28DRAFT_3178 Threonine dehydratase (Enterobacter asburiae PDN3) MHITYDLPVTIEDIQDARKRLAGKIYKTGMPRSNYLSERCKGEIFLKFENMQRTGSFKIR GAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGKVVMPMGAPKSKVAATRDY SAEVVLHGENFNDTIAKVSEIVEMEGRIFIPPYDDAKVIAGQGTIGLEILEDLYDVDNVI VPIGGGGLIAGIATAIKSINPTINIIGVQSENVHGMAASFQAGEIMSHRVTGTLADGCDV SRPGNLPFEIVRELVDDIVLVSEDNIRDSMIALIQRNKVVTEGAGALACAALLSGKLDHY IQGRKTVCIISGGNIDLSRVSQITGFVDA