Protein Info for EX28DRAFT_3175 in Enterobacter asburiae PDN3

Annotation: formate acetyltransferase 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01255: formate acetyltransferase
  • amino acids 15 to 763 (749 residues), 1280.4 bits, E=0
• PF02901: PFL-like
  • amino acids 30 to 618 (589 residues), 681.4 bits, E=1.6e-208
• PF01228: Gly_radical
  • amino acids 634 to 745 (112 residues), 120.3 bits, E=5.4e-39

Best Hits

Swiss-Prot: 90% identical to TDCE_ECOLI: PFL-like enzyme TdcE (tdcE) from Escherichia coli (strain K12)

KEGG orthology group: K00656, formate C-acetyltransferase [EC: 2.3.1.54] (inferred from 91% identity to cro:ROD_47631)

MetaCyc: 90% identical to 2-oxobutanoate formate-lyase/pyruvate formate-lyase 4 (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"2-ketobutyrate formate-lyase (EC 2.3.1.-) / Pyruvate formate-lyase (EC 2.3.1.54)" in subsystem Threonine anaerobic catabolism gene cluster or Butanol Biosynthesis or Fermentations: Mixed acid (EC 2.3.1.-, EC 2.3.1.54)

MetaCyc Pathways

• mixed acid fermentation (16/16 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (18/19 steps found)
• superpathway of N-acetylneuraminate degradation (19/22 steps found)
• superpathway of L-threonine metabolism (16/18 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (9/9 steps found)
• L-threonine degradation I (6/6 steps found)
• pyruvate fermentation to acetate IV (3/3 steps found)
• pyruvate fermentation to ethanol I (3/3 steps found)
• reductive monocarboxylic acid cycle (2/2 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)

KEGG Metabolic Maps

• 1- and 2-Methylnaphthalene degradation
• Alkaloid biosynthesis I
• Alkaloid biosynthesis II
• Anthocyanin biosynthesis
• Benzoate degradation via CoA ligation
• Biosynthesis of terpenoids and steroids
• Biosynthesis of type II polyketide backbone
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Carotenoid biosynthesis - General
• Diterpenoid biosynthesis
• Ether lipid metabolism
• Ethylbenzene degradation
• Fatty acid biosynthesis
• Glycerophospholipid metabolism
• Glycosphingolipid biosynthesis - ganglio series
• Histidine metabolism
• Limonene and pinene degradation
• Lipopolysaccharide biosynthesis
• Lysine degradation
• Phenylalanine metabolism
• Propanoate metabolism
• Pyruvate metabolism
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.-, 2.3.1.54

Use Curated BLAST to search for 2.3.1.- or 2.3.1.54

Protein Sequence (764 amino acids)

>EX28DRAFT_3175 formate acetyltransferase 1 (Enterobacter asburiae PDN3)
MKVTIDTGVAPYSDAWAGFRGEEWKNGVNVRDFIQHNYTPYEGDEAFLAQATPATTALWQ
KVMVGIRQENATHAPVDFDTNIATTITAHGPGYIDQDLETIVGLQTDKPLKRALHPYGGI
NMIRSSFEAYGREMDPQFEYLFTDLRKTHNQGVFDVYSPEMMRCRKSGVLTGLPDGYGRG
RIIGDYRRVALYGIAYLVRERELQFTDLQGRLERGEDLEATIRLREELAEHRRALLQIQQ
MAANYGFDISRPAMNAQEAVQWVYFAYLAAVKSQNGGAMSLGRTASFLDIYIERDMQAGR
LNEVQAQELIDHFIMKIRMVRFLRTPEFDTLFSGDPIWATEVIGGMGLDGRTLVTKNSFR
YLHTLHTMGPAPEPNLTILWSEQLPIAFKKYAAQVSIVTSSLQYENDDLMRADFNSDDYA
IACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTEPLLDDVLDYDTV
MASLDHFMDWLAVQYISALNLIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVAADS
LSAIKYATVKPVRDHTGLAVDFVIEGDYPQYGNNDDRVDSIACDLVERFMKKIQALPTYR
NAVPTQSILTITSNVVYGQKTGNTPDGRRGGTPFAPGANPMHGRDRKGAVASLTSVAKLP
FTYAKDGISYTFSIVPQALGKDELVRKTNLVGLLDGYFHHEASIEGGQHLNVNVMNREML
LDAIAHPENYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQSI