Protein Info for EX28DRAFT_3081 in Enterobacter asburiae PDN3

Annotation: Glutamine synthetase adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 951 PF03710: GlnE" amino acids 29 to 275 (247 residues), 327.6 bits, see alignment E=5e-102 amino acids 552 to 803 (252 residues), 353.5 bits, see alignment E=6.4e-110 PF08335: GlnD_UR_UTase" amino acids 296 to 435 (140 residues), 110.9 bits, see alignment E=5.2e-36 amino acids 824 to 917 (94 residues), 40.8 bits, see alignment E=2.3e-14

Best Hits

Swiss-Prot: 87% identical to GLNE_CITK8: Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (glnE) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 96% identity to enc:ECL_04379)

MetaCyc: 85% identical to fused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase (Escherichia coli K-12 substr. MG1655)
GSDEADENYLATION-RXN [EC: 2.7.7.89]; [Glutamate--ammonia-ligase] adenylyltransferase. [EC: 2.7.7.89, 2.7.7.42]

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42 or 2.7.7.89

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (951 amino acids)

>EX28DRAFT_3081 Glutamine synthetase adenylyltransferase (Enterobacter asburiae PDN3)
MPLSSPLQQQWQTVCERLPESLPASSLSEQAKSVLAFSDFVQESITANPDWLAELENAPP
QADEWRHYAGWLQTALEDVADEATLMRVLRQFRRRVMVRIAWAQSLELVSEESTLQQLSE
LAQTLIVAARDWLYAACCKEWGTPCSEEGVPQPLLILGMGKLGGCELNFSSDIDLIFAWP
ENGSTRGGRRELDNAQFFTRLGQRLIKALDQPTQDGFVYRVDMRLRPFGDSGPLVLSFAA
LEDYYQEQGRDWERYAMVKARIMGDSDDAYANELRAMLRPFVFRRYIDFSVIQSLRNMKG
MIAREVRRRGLKDNIKLGAGGIREIEFIVQVFQLIRGGREPSLQSRSLLPTLSAIEQLHL
LPDGDAQTLREAYLFLRRLENLLQSINDEQTQTLPGDDLNRARLAWGMRVDDWTALTERL
DAHMAGVRRIFNELIGDDESESQDDALSEHWRELWQDALQEDDTTPVLTHLTDDARHRVV
ALIADFRLELNKRAIGPRGRQVLDHLMPHLLSEVCSRADAPVPLSRMMPLLSGIITRTTY
LELLSEFPGALKHLITLCAASPMVANKLARYPLLLDELLDPNTLYQPTATDAYRDELRQY
LLRVPEEDEEQQLEALRQFKQAQMLRVAAADIAGTLPVMKVSDHLTWLAEAIIDAVVHQA
WVQMVARYGQPKHLAEREGRGFAVVGYGKLGGWELGYSSDLDLIFLHDCPVDVMTDGERE
IDGRQFYLRLAQRIMHLFSTRTSSGILYEVDARLRPSGAAGMLVTSTESFADYQKNEAWT
WEHQALVRARVVYGDPQLKTQFDVIRREVMTTLRDGSTLQTEVREMREKMRAHLGNKHRD
RFDIKADEGGITDIEFITQYLVLLHAHDKPKLTRWSDNVRILELLAQNDIMDEQEAQALT
RAYTTLRDELHHLALQEEPGHVALDSFAHERAQVTASWQKWLVEPCVTNQV