Protein Info for EX28DRAFT_3080 in Enterobacter asburiae PDN3

Annotation: rfaE bifunctional protein, domain I/rfaE bifunctional protein, domain II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 476 TIGR02198: bifunctional protein RfaE, domain I" amino acids 5 to 312 (308 residues), 425.3 bits, see alignment E=2e-131 PF00294: PfkB" amino acids 13 to 305 (293 residues), 189.1 bits, see alignment E=1.9e-59 PF08543: Phos_pyr_kin" amino acids 189 to 288 (100 residues), 32.6 bits, see alignment E=8.4e-12 TIGR02199: bifunctional protein RfaE, domain II" amino acids 332 to 473 (142 residues), 215.5 bits, see alignment E=3.9e-68 TIGR00125: cytidyltransferase-like domain" amino acids 342 to 408 (67 residues), 62.9 bits, see alignment E=3.6e-21 PF01467: CTP_transf_like" amino acids 344 to 469 (126 residues), 67.9 bits, see alignment E=1.6e-22

Best Hits

Swiss-Prot: 97% identical to HLDE_ENT38: Bifunctional protein HldE (hldE) from Enterobacter sp. (strain 638)

KEGG orthology group: K03272, D-beta-D-heptose 7-phosphate kinase / D-beta-D-heptose 1-phosphate adenosyltransferase [EC: 2.7.1.- 2.7.7.-] (inferred from 98% identity to enc:ECL_04378)

MetaCyc: 95% identical to fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-4342 [EC: 2.7.7.70]; RXN0-4341 [EC: 2.7.7.70, 2.7.1.167]

Predicted SEED Role

"ADP-heptose synthase (EC 2.7.-.-) / D-glycero-beta-D-manno-heptose 7-phosphate kinase" in subsystem LOS core oligosaccharide biosynthesis (EC 2.7.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.-, 2.7.7.-

Use Curated BLAST to search for 2.7.-.- or 2.7.1.- or 2.7.1.167 or 2.7.7.- or 2.7.7.70

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (476 amino acids)

>EX28DRAFT_3080 rfaE bifunctional protein, domain I/rfaE bifunctional protein, domain II (Enterobacter asburiae PDN3)
MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVDTIEERPGGAANVAMNI
ASLGAQSRLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHPTITKLRVLSRNQQLIRLD
FEEGFEGVDPEPLHERINQALGNIGALVLSDYAKGALASVKTMIQLARKANVPVLIDPKG
TDFERYRGATLLTPNLSEFEAVAGKCKTEEELVERGMKIIADFELSALLVTRSEQGMTLL
QPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAAGNSLEEACYFANAAAGVVVGKLGT
STVSPIELENAVRGRADTGFGVMTEDELKVAVAAARKRGEKVVMTNGVFDILHAGHVSYL
ANARKLGDRLIVAVNSDASTKRLKGETRPVNPLEQRMIVLGALEAVDWVVSFEEDTPQRL
IAGILPDLLVKGGDYKPEQIAGSEEVWANGGEVMVLNFEDGCSTTNIIKKIQKDSQ