Protein Info for EX28DRAFT_3031 in Enterobacter asburiae PDN3

Annotation: Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 298 PF00561: Abhydrolase_1" amino acids 37 to 189 (153 residues), 73.7 bits, see alignment E=1.9e-24 PF12697: Abhydrolase_6" amino acids 38 to 283 (246 residues), 52.1 bits, see alignment E=1.5e-17

Best Hits

KEGG orthology group: None (inferred from 81% identity to spe:Spro_2543)

Predicted SEED Role

"Epoxide hydrolase (EC 3.3.2.9)" (EC 3.3.2.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.3.2.9

Use Curated BLAST to search for 3.3.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (298 amino acids)

>EX28DRAFT_3031 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) (Enterobacter asburiae PDN3)
MEQQYGEIAHADLRAEHVYTKVNGQRIHCVVAGKGQPVLLLPGWPQTWYTWRYVLQALAA
AGFQAIAVDPPGIGESDKPRDGYDTGRVGAMLHTMMAQLGHARYSIVGHDIGMWVGYAMA
SDFPQAIERLVLTEAVIPGLAPPPPIFVAPEENIFLWHFMFNQLADLPETLTSGRESQYL
SYIFDRWSHRRERVAAEVYIAAYSTPGGLRAGFDYYRAIPETIRQNQRRAQTPLTMPVLT
VGAEHATRDAPLITLQGNALDLRGETVADCGHFITEECHEAFIGLIVPFLSEDTDRAA