Protein Info for EX28DRAFT_3018 in Enterobacter asburiae PDN3

Annotation: poly-beta-1,6 N-acetyl-D-glucosamine export porin PgaA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 812 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details TIGR03939: poly-beta-1,6 N-acetyl-D-glucosamine export porin PgaA" amino acids 18 to 812 (795 residues), 849.6 bits, see alignment E=1.2e-259 PF21197: PgaA_barrel" amino acids 519 to 812 (294 residues), 422.2 bits, see alignment E=5.1e-131

Best Hits

KEGG orthology group: K11935, biofilm PGA synthesis protein PgaA (inferred from 80% identity to enc:ECL_04313)

Predicted SEED Role

"Biofilm PGA outer membrane secretin PgaA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (812 amino acids)

>EX28DRAFT_3018 poly-beta-1,6 N-acetyl-D-glucosamine export porin PgaA (Enterobacter asburiae PDN3)
MESSLRFNTLFFYRTPTLLFLLFLFPVLAQATESVYEQQIQQARNGNYTPFLDYLQRYQQ
QHALTPAQVADWLQVAAWAGHDDEVIQVWQRYGIYMPLPARGVAAVAQSRRNQKAWQPAL
ARWKEARSLAPDNDDYRIGYIKTLADARMDTLALQEARRLVAENPSQAHLQTLSYVWLRQ
GKSWDRLLADTRALNIAPDNKALLNELIDALTDSRVNTPALQLSQNVTLSPAERRRLERN
AAAERVRLADVPGRTEKERLQLAQSALNRYDALLARWQNEPQAAEDVVLARIDRLGALYA
HADYPQVISEYQDLTASQHPVPGWAIGWIISAYLQEQNAAAAYALLQRYPQYASDPEDEE
HALFYAWLDTGDYQSARQYVERQTRNVPWTRYDFGSPTPQPNDRWLTGQSLRFNYLLATN
ALPEAETLAHRLATTAPGNQGLQIDYATLLQARGLPRAAEQKLKKAEALEPSNTELEQQQ
AYVAMDLQEWRQMDLLADDVLARAPNDRSARRLDRLRTVHHMSELRLNASKGLHSDSPVS
GMHDLSWDATLYGPPVADSWRLFAGTRYAQSNFDEGKGTSRHLLGGVEWRPRDLQLEAEL
SSNRYHGENKPGARLATTYFVTDSWQVSGSLERLSRTTPLRALRNGISANRGEGGVRWYQ
NERREYQFSAALSRFSDRNRRQEYILTGKERLWQTPSLTLDLEPGIAASKNSLRDTLYYN
PARDLSVTAALAVDHEMYRHYDTLWSQQFVAGGGSYWQKNQSAGAIALLGYGQRVQWNNV
IDTGVMLNWDKRPYDGKRESNLSVTFDATLRF