Protein Info for EX28DRAFT_3009 in Enterobacter asburiae PDN3

Annotation: Na+/citrate symporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 453 transmembrane" amino acids 33 to 53 (21 residues), see Phobius details amino acids 63 to 81 (19 residues), see Phobius details amino acids 87 to 109 (23 residues), see Phobius details amino acids 127 to 148 (22 residues), see Phobius details amino acids 155 to 178 (24 residues), see Phobius details amino acids 184 to 211 (28 residues), see Phobius details amino acids 221 to 242 (22 residues), see Phobius details amino acids 279 to 297 (19 residues), see Phobius details amino acids 302 to 322 (21 residues), see Phobius details amino acids 334 to 355 (22 residues), see Phobius details amino acids 362 to 387 (26 residues), see Phobius details amino acids 431 to 452 (22 residues), see Phobius details PF03390: 2HCT" amino acids 36 to 444 (409 residues), 464 bits, see alignment E=1.9e-143

Best Hits

Swiss-Prot: 37% identical to MAEN_BACSU: Na(+)-malate symporter (maeN) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 98% identity to enc:ECL_04300)

Predicted SEED Role

"Malate Na(+) symporter" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (453 amino acids)

>EX28DRAFT_3009 Na+/citrate symporter (Enterobacter asburiae PDN3)
MSTTDDSFSVTHDPIEIQRPSLKERWWHIMDTWKVGIIPLPLFVLAGALIAIDCLGGKLP
SDIVVMVATLAFFGFACGEFGKRLPIVGKLGAAAICATFIPSALVYYGLLPDVVVESTTK
FYKSTNILYLYICCIIVGSIMSMNRTVLIQGFLRIFFPMLCGEIVGMLVGMGVGLALGLE
PFQIFFFIILPIMAGGVGEGAIPLSIGYATLLHMDQGVALGRVLPMVMLGGLTAIIISGC
LNQLGKRFPHLTGEGQLMPNRANADATVSQPAFSGKADVTTIASGALLAVLLYMLGMLGH
KLIGLPAPVGMLFMAVLVKLCNGASPRLLEGSQVVYKFFQTSVTYPILFAVGVAITPWHE
LVAAFTVSNLLVIVSTVSALVATGFFVGKKIGMHPIDVAIVSCCQSGQGGTGDVAILTAG
NRMSLMPFAQIATRIGGAINVSVSLLILGNFLV