Protein Info for EX28DRAFT_3008 in Enterobacter asburiae PDN3

Annotation: 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 287 PF01557: FAA_hydrolase" amino acids 74 to 276 (203 residues), 212.9 bits, see alignment E=2.4e-67

Best Hits

KEGG orthology group: None (inferred from 98% identity to enc:ECL_04299)

Predicted SEED Role

"Fumarylacetoacetate hydrolase family protein" in subsystem Gentisare degradation or Salicylate and gentisate catabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (287 amino acids)

>EX28DRAFT_3008 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) (Enterobacter asburiae PDN3)
MKLASFLYQGKRSYGIVQADGVIDLGRRLGDRYSDLKALLQGNGLAQATRYLNDAVDVPM
SAITFLPVIEQPEKILCVGMNYADKRKEFDQHNPAPTLFVRFPDSQTGHNEPVLKPRHSS
EFDYEGELAVIIGKGGENISRDDALRHVAGYSCYMDGSARDWQHTWFTAGKNWRKTGAFG
PWMATADEIPDPHQLAIRTWLNGRMVQEDNTSSMIHKVAELIEYISTFTRLSPGDVIITG
SPGGVGKKRNPPLFMKEGDRIEVEIEHIGHLSNVIVEAPAVGLAAAH