Protein Info for EX28DRAFT_3004 in Enterobacter asburiae PDN3

Annotation: citrate lyase, alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 507 TIGR01584: citrate lyase, alpha subunit" amino acids 35 to 505 (471 residues), 760.9 bits, see alignment E=2.8e-233 PF04223: CitF" amino acids 38 to 503 (466 residues), 797.8 bits, see alignment E=1.9e-244

Best Hits

Swiss-Prot: 68% identical to CILA_KLEPN: Citrate lyase alpha chain (citF) from Klebsiella pneumoniae

KEGG orthology group: K01643, citrate lyase subunit alpha / citrate CoA-transferase [EC: 2.8.3.10 4.1.3.6] (inferred from 98% identity to enc:ECL_04295)

MetaCyc: 68% identical to citrate lyase alpha subunit (Klebsiella pneumoniae)
Citrate (pro-3S)-lyase. [EC: 4.1.3.6]; Citrate CoA-transferase. [EC: 4.1.3.6, 2.8.3.10]

Predicted SEED Role

"Citrate lyase alpha chain (EC 4.1.3.6)" (EC 4.1.3.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.6

Use Curated BLAST to search for 2.8.3.10 or 4.1.3.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (507 amino acids)

>EX28DRAFT_3004 citrate lyase, alpha subunit (Enterobacter asburiae PDN3)
MNQTELLHVNFPHLRDLKPFDTAHSATPWLADSDAKHSRKLCASVEEAVMRSGLQDGMTI
SFHHAFREGDRVINTVVALLARMGFKNLTLASSSLMTCNDALIEHIESGVITRIYTSGMR
GRLADAISHGLMDEPVQIHSHGGRVKLLQDGELNIDVAFLGVPCSDEFGNANGTHGKSCC
GSLGYAMVDAHFARKVVLLTEELVPFPNMPASLVQDQVDYIVQVESVGDPAKISVGAARV
TSNPRELMIARYAADVIEHSGYFKPGFSMQTGSGAAATACTRFMEEKMERSGVKARFALG
GITGSLVDLHEKGLIEKLLDTQCFDGQAAASLARNPNHVEISTNVYANPGSKAASCDQLD
VVILSALEIDVDFNVNVITGSDGVMRGASGGHCDVAAAANLTIVVAPLLRSRIPTVVKRV
TTRLTPGESIDVLVTDHGIAVNPARPEIRERLLEAGLKVVDISALYERAISLTGVPKPIE
FTDKIVGVIRYRDGSVIDTVRQVKEEV