Protein Info for EX28DRAFT_3000 in Enterobacter asburiae PDN3

Annotation: Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 359 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF11873: Mltc_N" amino acids 31 to 191 (161 residues), 236.7 bits, see alignment E=1.1e-74 PF01464: SLT" amino acids 195 to 319 (125 residues), 111.9 bits, see alignment E=1.3e-36

Best Hits

Swiss-Prot: 95% identical to MLTC_ENT38: Membrane-bound lytic murein transglycosylase C (mltC) from Enterobacter sp. (strain 638)

KEGG orthology group: K08306, membrane-bound lytic murein transglycosylase C [EC: 3.2.1.-] (inferred from 97% identity to enc:ECL_04290)

MetaCyc: 90% identical to membrane-bound lytic murein transglycosylase C (Escherichia coli K-12 substr. MG1655)
4.2.2.f [EC: 4.2.2.f]; 4.2.2.f [EC: 4.2.2.f]

Predicted SEED Role

"Membrane-bound lytic murein transglycosylase C precursor (EC 3.2.1.-)" (EC 3.2.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.- or 4.2.2.f

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (359 amino acids)

>EX28DRAFT_3000 Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains) (Enterobacter asburiae PDN3)
MMKKLLALALVAPLLVSCSSKKGEDYNEAWIKDTNGFDILMGQFAHNIENIWGFNEVLIA
GPKDYVKYTDAYQTRSHINFDEGTITVETIAGTDPAARLRQAIIKTLLMGDDPGSIDLYS
DADDITISKEPFLYGQVVDQTGQPIRWEGRATKFADYLLQTRLKSRSNGLKIIYSVTINL
VPNHLDKRAHKYIGMVRQASRKYGVDESLILAIMQTESSFNPYAVSRSDALGLMQVVQHS
AGKDVFRAQGKSGTPSRSYLFDPQSNIDTGTAYLAMLNNVYLGGIDNPTSRRYAVITAYN
GGAGSVLRVFSNDKVQAANIINSMAPGDVYSTLTTRHPSAESRRYLYKVNTAQKNYRRR