Protein Info for EX28DRAFT_2897 in Enterobacter asburiae PDN3

Annotation: DNA helicase/exodeoxyribonuclease V, gamma subunit (EC 3.1.11.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1124 TIGR01450: exodeoxyribonuclease V, gamma subunit" amino acids 2 to 1089 (1088 residues), 1434.3 bits, see alignment E=0 PF04257: Exonuc_V_gamma" amino acids 2 to 346 (345 residues), 370.8 bits, see alignment E=5.6e-115 PF17946: RecC_C" amino acids 832 to 1051 (220 residues), 225.5 bits, see alignment E=5.9e-71

Best Hits

Swiss-Prot: 79% identical to RECC_ECOLI: RecBCD enzyme subunit RecC (recC) from Escherichia coli (strain K12)

KEGG orthology group: K03583, exodeoxyribonuclease V gamma subunit [EC: 3.1.11.5] (inferred from 93% identity to enc:ECL_04145)

MetaCyc: 79% identical to exodeoxyribonuclease V subunit RecC (Escherichia coli K-12 substr. MG1655)
Exodeoxyribonuclease V. [EC: 3.1.11.5]; RXN-19004 [EC: 3.1.11.5]

Predicted SEED Role

"Exodeoxyribonuclease V gamma chain (EC 3.1.11.5)" in subsystem DNA-replication (EC 3.1.11.5)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.5

Use Curated BLAST to search for 3.1.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1124 amino acids)

>EX28DRAFT_2897 DNA helicase/exodeoxyribonuclease V, gamma subunit (EC 3.1.11.5) (Enterobacter asburiae PDN3)
MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMVLVQSTGMAQWLQMSLSRKFGIAANI
DFPLPASFIWEMFVRVLPDIPEQSAFNKQGMSWKLMALLPEMLQHDEFAMLRHYLNDDTD
KRKLFQLASRTADLYDQYLVYRSDWLIRWEAGELVEGLPEAQIWQAPLWKALVEHTEKLG
QPKWHRANLYDRFISILENAPERPARLPSRVFICGISALPPVYLKALNALGKHIDIHILF
TNPCRQYWGDILDERWLARLVTRQRKRLFEERSVPLFKDGSTAGQLFDEDGIQNLPNPLL
ASWGKLGRDYIHMLSDITSSGEGDVDAFVDITPDSLLHHIQLDILDLENRAVMGVTAKEF
ERSDSKRKLDADDRSITIHVCHSPQREVEVLHDQLLAMLQDDPELTPRDIVVMVADIDSY
SPFIQAVFGSATGERYLPYAISDRRARQSHPALQAFITLLSLPDSRFISEDVLALLDVPV
LAARFNINEEGLRYLRQWVNESGVRWGIDDDNVQEFELPPTGQHTWRFGLTRMLLGYAME
SSQGEWNEVLPYDESSGLIAELVGHLASLLMQLNRWRHELMQPRPLEAWLPVCRALLNDF
FLPDSDTEAAMALIETQWQAIIDEGVNSHYQEAIPLSLLRDELTQRLDQERISQRFLAGP
INICTLMPMRSIPFKVVCLLGMNDGVYPRALAPLGFDLMSADPKRGDRSRRDDDRYLFLE
ALISAQSKLYISYIGRSIQDNSERFPSVLVQELVDYIGQSHYLPGDEERNCDESEQRVKA
HITCFHSRMPFDPVNYTASERQSYAQEWLPAAKKEGSAHTDFIQELDPRPVDTLTFEQLQ
RFWAHPVRAFFQQRLQVNFRSEESEIPDAEPFTLEGLERYQLNLQLLNALVEEEDADKLY
RRYRAAGQLPYGAFGEIVWEAQCQEMTALAERVRECRQPGKSIEIDLNCNGVQLTGWLTQ
VQPDGLLRWRPSMLSVSQGLQLWLEHLVYSADGHKGESRIFVRKEGEWRFPPMESEQALR
YLSLHIEGYRQGMNKPLLLLPESGGAWIKACYDAQNDAMLTDEASLQKARSKFMQAYEGN
MMVRGEGEDVWYQRLWRTLEPEYFEAITEEAQRYLLPLFKFNQS