Protein Info for EX28DRAFT_2895 in Enterobacter asburiae PDN3

Annotation: DNA helicase/exodeoxyribonuclease V, beta subunit (EC 3.1.11.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1180 TIGR00609: exodeoxyribonuclease V, beta subunit" amino acids 8 to 1172 (1165 residues), 1410.9 bits, see alignment E=0 PF00580: UvrD-helicase" amino acids 13 to 436 (424 residues), 167.7 bits, see alignment E=1.1e-52 PF13245: AAA_19" amino acids 378 to 423 (46 residues), 25.5 bits, see alignment (E = 3.2e-09) PF13361: UvrD_C" amino acids 442 to 808 (367 residues), 66.7 bits, see alignment E=6.7e-22 PF12705: PDDEXK_1" amino acids 951 to 1151 (201 residues), 24.2 bits, see alignment E=7.8e-09

Best Hits

Swiss-Prot: 78% identical to RECB_ECOLI: RecBCD enzyme subunit RecB (recB) from Escherichia coli (strain K12)

KEGG orthology group: K03582, exodeoxyribonuclease V beta subunit [EC: 3.1.11.5] (inferred from 93% identity to enc:ECL_04143)

MetaCyc: 78% identical to exodeoxyribonuclease V subunit RecB (Escherichia coli K-12 substr. MG1655)
RXN-11135 [EC: 5.6.2.4]; Exodeoxyribonuclease V. [EC: 5.6.2.4, 3.1.11.5]; RXN-19004 [EC: 5.6.2.4, 3.1.11.5]

Predicted SEED Role

"Exodeoxyribonuclease V beta chain (EC 3.1.11.5)" in subsystem DNA-replication (EC 3.1.11.5)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.5

Use Curated BLAST to search for 3.1.11.5 or 5.6.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1180 amino acids)

>EX28DRAFT_2895 DNA helicase/exodeoxyribonuclease V, beta subunit (EC 3.1.11.5) (Enterobacter asburiae PDN3)
MTDTAESLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGLGGNAAFPRPLSVEELL
VVTFTEAATAELRGRIRSNIHELRIACLRQSTDNPLYASLLEEIADKQQAAQWLLLAERQ
MDEASVFTIHGFCQRMLSLNAFESGMLFEQQLIEDESVLRYQACADFWRRHCYPLQRDIA
EAVHALWKGPEALLRAIDRYLQGEAPVIKSPPPADETLASRHEKIIARIADIKQKWIESV
GEIDVIIENSGIDRRKFNRGNQGKWIDKISAWAQEETRGYQLPDALEKFSRRFLVERTKA
DGIVPEHPLFVAIETLLAEPLTLNDLMITRAMTEIRDAVAREKRRRGELGFDDMLSRLDA
ALCSENGEALAAAIRTRFPVAMIDEFQDTDPQQYRIFRRIWRQQPGTALLLIGDPKQAIY
AFRGADIFTYMKARSEVDAHYTLDTNWRSSPGMVESVNALFSRMDTAFMFREIPFQPVKF
AEKNASLRFEFNGETQPAMNFWLLDGEGYGVADYQNAMAQHCAAQIRDWLSAGAHGKALL
WKGDKPAPVKASDITVLVRSRQEAALIRDALTLLNIPSVYLSNRDSVFETLEAQEMLWLL
QAVLAPERESTLRSALASAMLGLNARDIDALNNDEAAWDAVVEEFVRYREKWQKRGVMAM
VRELMAQRHIAENMLATAGGERRLTDILHISELLQEAGTQLESEHALVRWLAQQIADPNS
NSSSQQMRLESDKHLVQIVTIHKSKGLEYPLVWLPFIANYRVQDQAFYHDRESFEAVLDL
SNAESSLELAEAERLAEDLRLLYVALTRSVWHCSLGVAPLFRRRGEKTGESDFHLSALGR
LIQHGEPKDAAGLRQCIESLCTEHVALHIPSQPDNSRWQMPELRDSQLHARQVVRTIADD
WRVTSYSGLQQHGQSIAQDLMPKLDVDAAGAGSVPAEPVLTPHQFPRGASPGTFLHSLFE
ELDFTQPVSEEWVLKMLQSGGYDAQWQPVLTDWIRAVLHAPLTTQGISLNQLTAKDKQVE
MEFYLPIASPLRAEALDALIREYDPLSAGCPPLNFRQVQGMLKGFIDLVFRHEGRYYLLD
YKSNWLGESSEAYTQDAMASAMQMHRYDLQYQLYTLALHRYLRHRMADYRYEDHFGGVIY
LFLRGVDANDPRSGVFSTRPAAELIEKMDNLFAASTEEVA