Protein Info for EX28DRAFT_2894 in Enterobacter asburiae PDN3

Annotation: DNA helicase/exodeoxyribonuclease V, alpha subunit (EC 3.1.11.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 606 PF21185: RecD_N" amino acids 7 to 107 (101 residues), 113.7 bits, see alignment E=2.2e-36 TIGR01447: exodeoxyribonuclease V, alpha subunit" amino acids 9 to 599 (591 residues), 641.2 bits, see alignment E=8.6e-197 PF13086: AAA_11" amino acids 153 to 214 (62 residues), 27.3 bits, see alignment E=1.6e-09 PF13245: AAA_19" amino acids 153 to 303 (151 residues), 120.8 bits, see alignment E=2.3e-38 PF13604: AAA_30" amino acids 154 to 315 (162 residues), 96 bits, see alignment E=1.2e-30 PF00580: UvrD-helicase" amino acids 156 to 240 (85 residues), 29.1 bits, see alignment E=4.2e-10 PF13401: AAA_22" amino acids 162 to 284 (123 residues), 28.8 bits, see alignment E=7.1e-10 PF09848: SLFN-g3_helicase" amino acids 164 to 271 (108 residues), 22 bits, see alignment E=4.6e-08 PF05127: Helicase_RecD" amino acids 167 to 284 (118 residues), 48.4 bits, see alignment E=5.4e-16 PF01443: Viral_helicase1" amino acids 530 to 579 (50 residues), 24.4 bits, see alignment 1.2e-08 PF13538: UvrD_C_2" amino acids 532 to 578 (47 residues), 48.3 bits, see alignment 3.6e-16

Best Hits

Swiss-Prot: 73% identical to RECD_ECOLI: RecBCD enzyme subunit RecD (recD) from Escherichia coli (strain K12)

KEGG orthology group: K03581, exodeoxyribonuclease V alpha subunit [EC: 3.1.11.5] (inferred from 92% identity to enc:ECL_04142)

MetaCyc: 73% identical to exodeoxyribonuclease V subunit RecD (Escherichia coli K-12 substr. MG1655)
RXN0-4261 [EC: 5.6.2.3]; Exodeoxyribonuclease V. [EC: 5.6.2.3, 3.1.11.5]; RXN-19004 [EC: 5.6.2.3, 3.1.11.5]

Predicted SEED Role

"Exodeoxyribonuclease V alpha chain (EC 3.1.11.5)" in subsystem DNA-replication (EC 3.1.11.5)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.5

Use Curated BLAST to search for 3.1.11.5 or 5.6.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (606 amino acids)

>EX28DRAFT_2894 DNA helicase/exodeoxyribonuclease V, alpha subunit (EC 3.1.11.5) (Enterobacter asburiae PDN3)
MTMQALLLDAVEQRLLRHLDVQFAMMVAGEEPAVMLAAAILSKDAGEGHVCLPLSRLAVD
EKMPPALQACFALLGDAVDWQEILLGSPAVSGADTGTPMILVGERLYLNRLWRNELTVAR
FFSETNAPLPCDEAQLRQTLDTLFTSDDATDWQKVAAAVALTRRISVISGGPGTGKTTTV
AKLLAALIQLSSEQKCRIRLAAPTGKAAARLTESLGGALQKLPLTREQLALFPNEASTLH
RLLGAQPGSQRLRYHAGNPLHLDVLVVDEASMIDLTMMSRLIDALPPHARVIFLGDRDQL
ASVEAGAVLGDICTYASLGYTAERAQELARLTGCSLTSENHSLAGALRDSLCLLQKSYRF
GSDSGIGQLAAAVNRGDRRTTTAVFDGTFTDIEKKSLQSGEEYQAMLDDALQGYQHFLTG
VQQQSTPEQVIAAFGEYQLLCALREGPFGVRGLNDRLEQLLAQKRKINRTPHSRWYEGRP
VMISRNDSTLGLFNGDIGIALDCGQGLRVWFQMPDGSVKSFQPSRLPEHETAWAMTVHKS
QGSEFNHAALILPTQLSPVITRELIYTAITRARQRLSLYADERVLVQAIATRTERRSGLG
AIFESL