Protein Info for EX28DRAFT_2872 in Enterobacter asburiae PDN3

Annotation: Pseudouridylate synthases, 23S RNA-specific

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00849: PseudoU_synth_2
  • amino acids 12 to 171 (160 residues), 106.6 bits, E=7.3e-35

Best Hits

Swiss-Prot: 89% identical to TRUC_SHIFL: tRNA pseudouridine synthase C (truC) from Shigella flexneri

KEGG orthology group: K06175, tRNA pseudouridine synthase C [EC: 5.4.99.12] (inferred from 96% identity to enc:ECL_04124)

MetaCyc: 89% identical to tRNA pseudouridine65 synthase (Escherichia coli K-12 substr. MG1655)
RXN-11840 [EC: 5.4.99.26]

Predicted SEED Role

"tRNA pseudouridine synthase C (EC 4.2.1.70)" (EC 4.2.1.70)

MetaCyc Pathways

• pseudouridine degradation (1/2 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12 or 5.4.99.26

Protein Sequence (258 amino acids)

>EX28DRAFT_2872 Pseudouridylate synthases, 23S RNA-specific (Enterobacter asburiae PDN3)
MTLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIGQHVFTAHRLDRPTSG
VLLMGLSSEAGRLLSQQFEQHQMQKRYHAIVRGWLTEAATLDYPLVEELDKIADKFARDD
KGPQPAVTDYRGMATTEMPVATSKFPTTRYSLVELLPKTGRKHQLRRHLAHLRHPIIGDS
KHGDLRQNRSAAEHFGCHRLMLHASELSLTHPFTGEPLTIRAGLDDVWMQALSQFGWLGQ
LPENERVEFVSGNVQDER