Protein Info for EX28DRAFT_2818 in Enterobacter asburiae PDN3

Annotation: hydrogenase accessory protein HypB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00073: hydrogenase accessory protein HypB
  • amino acids 73 to 286 (214 residues), 313.4 bits, E=3.4e-98
• PF02492: cobW
  • amino acids 103 to 263 (161 residues), 81.2 bits, E=3.9e-27

Best Hits

Swiss-Prot: 85% identical to HYPB_ECOL6: Hydrogenase maturation factor HypB (hypB) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K04652, hydrogenase nickel incorporation protein HypB (inferred from 95% identity to enc:ECL_04067)

MetaCyc: 85% identical to hydrogenase isoenzymes nickel incorporation protein HypB (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]; RXN-22957 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"[NiFe] hydrogenase nickel incorporation-associated protein HypB" in subsystem NiFe hydrogenase maturation

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• NiFe(CO)(CN)₂ cofactor biosynthesis (9/10 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Protein Sequence (287 amino acids)

>EX28DRAFT_2818 hydrogenase accessory protein HypB (Enterobacter asburiae PDN3)
MCSTCGCAEGNLYIEGDEHRPHSAFRSAPFSPAPRPSAALTGITFAPQRSAAGDLHYGHG
EAGTHAPGISQRQMLEVEINVLDKNNQIAARNRARFAARKQLVLNLVSSPGSGKTTLLTE
TLKRLNGRVSCAVIEGDQQTVNDAARIRETGTPAIQVNTGKGCHLDAQMIADAAPRLPLA
DNGILFIENVGNLVCPASFDLGERHKVAVLSVTEGEDKPLKYPHMFAAASLMLLNKVDLL
PYLNFDVDKCLAYAREVNPEIEILLVSATRGDGMDAWLNWLENERCA