Protein Info for EX28DRAFT_2797 in Enterobacter asburiae PDN3

Annotation: KpsF/GutQ family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 321 PF01380: SIS" amino acids 39 to 170 (132 residues), 109.4 bits, see alignment E=1.1e-35 TIGR00393: sugar isomerase, KpsF/GutQ family" amino acids 43 to 311 (269 residues), 426.4 bits, see alignment E=2.5e-132 PF00571: CBS" amino acids 203 to 256 (54 residues), 29.8 bits, see alignment 6.2e-11 amino acids 265 to 317 (53 residues), 29.8 bits, see alignment 6.2e-11

Best Hits

Swiss-Prot: 88% identical to GUTQ_SHIFL: Arabinose 5-phosphate isomerase GutQ (gutQ) from Shigella flexneri

KEGG orthology group: K02467, GutQ protein (inferred from 94% identity to enc:ECL_04045)

MetaCyc: 87% identical to D-arabinose 5-phosphate isomerase GutQ (Escherichia coli K-12 substr. MG1655)
Arabinose-5-phosphate isomerase. [EC: 5.3.1.13]

Predicted SEED Role

"D-arabinose 5-phosphate isomerase (EC 5.3.1.13)" (EC 5.3.1.13)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 5.3.1.13

Use Curated BLAST to search for 5.3.1.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (321 amino acids)

>EX28DRAFT_2797 KpsF/GutQ family protein (Enterobacter asburiae PDN3)
MSDFLLETGRQTLMLELQEASRLPERLGEDFVRAANTIIHCEGKVIVAGIGKSGHIGKKI
AATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGSAKELDLIIPRLQEKSVALL
AMTGKSRSPLALAAKATLDISVEREACPMHLAPTSSTVNTLMMGDALAMAVMQARGFNEE
DFARSHPAGALGARLLNKVHHLMRTDDAIPQVKLDTSVMDAMLELSRTGLGLVAVCDDNG
SVKGVFTDGDLRRWLVGGGRLETNVSDAMTKGGLTLNAESRAIEAKEVLMKRKITAAPVV
DDAGRLCGAINLQDFYQAGII