Protein Info for EX28DRAFT_2774 in Enterobacter asburiae PDN3

Annotation: glutamate-cysteine ligase (EC 6.3.2.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 514 TIGR01434: glutamate--cysteine ligase" amino acids 6 to 512 (507 residues), 896.9 bits, see alignment E=1.8e-274 PF04262: Glu_cys_ligase" amino acids 10 to 376 (367 residues), 568 bits, see alignment E=3.5e-175

Best Hits

Swiss-Prot: 93% identical to GSH1_ENT38: Glutamate--cysteine ligase (gshA) from Enterobacter sp. (strain 638)

KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 97% identity to enc:ECL_04027)

MetaCyc: 86% identical to glutamate--cysteine ligase (Escherichia coli K-12 substr. MG1655)
Glutamate--cysteine ligase. [EC: 6.3.2.2]

Predicted SEED Role

"Glutamate--cysteine ligase (EC 6.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 6.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (514 amino acids)

>EX28DRAFT_2774 glutamate-cysteine ligase (EC 6.3.2.2) (Enterobacter asburiae PDN3)
MIPDVSQALAWLENHPQALKGIQRGLERETLRVNADGSLATTGHPKALGSALTHKWITTD
FAEALLEFITPVEGDIDHMLTIMRDIHRYTARNMGDERMWPLSMPCYIEQGQEIELAQYG
TSNIGRLKTLYREGLKNRYGALMQTISGVHYNFSLPMAFWQAKCGETDKDAISEGYFRLI
RNYYRFGWVIPYLFGASPAICSSFLQGKPTTLPFEKTECGMYYLPYATSLRLSDLGYTNK
SQSNLGITFNELHEYVAGLKRAIKTPSEEYEKIGLEKDGKRLQINSNVLQIENELYAPIR
PKRVTRSGETPSDALQRGGIEYIEVRSLDINPFSPIGVDEQQIRFLDLFMVWCVLADAPE
MSSDELLCTRTNWNRVILEGRKPGLTLGIGCETAQFPLTKVGKDLFRDLKRVARTMDSID
GGDAYQQICDQLVECFDNPELTFSARILRSMIDQGIGGTGRSLSAEYREMLMQEPLEVLS
EADFAAERDASVVRQKEVEAADTESFEAFLAKQA