Protein Info for EX28DRAFT_2761 in Enterobacter asburiae PDN3

Annotation: ribonucleoside-diphosphate reductase, alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR04170: ribonucleoside-diphosphate reductase, class 1b, alpha subunit
  • amino acids 15 to 714 (700 residues), 1241.3 bits, E=0
• PF08343: RNR_N
  • amino acids 16 to 97 (82 residues), 96.5 bits, E=1.1e-31
• PF00317: Ribonuc_red_lgN
  • amino acids 99 to 171 (73 residues), 58.9 bits, E=6.6e-20
• TIGR02506: ribonucleoside-diphosphate reductase, alpha subunit
  • amino acids 104 to 696 (593 residues), 518.5 bits, E=2.5e-159
• PF02867: Ribonuc_red_lgC
  • amino acids 176 to 694 (519 residues), 533.1 bits, E=1.2e-163

Best Hits

Swiss-Prot: 89% identical to RIR3_ECOLI: Ribonucleoside-diphosphate reductase 2 subunit alpha (nrdE) from Escherichia coli (strain K12)

KEGG orthology group: K00525, ribonucleoside-diphosphate reductase alpha chain [EC: 1.17.4.1] (inferred from 96% identity to enc:ECL_04015)

MetaCyc: 89% identical to ribonucleoside-diphosphate reductase 2 subunit alpha (Escherichia coli K-12 substr. MG1655)
Ribonucleoside-diphosphate reductase. [EC: 1.17.4.1]; 1.17.4.1 [EC: 1.17.4.1]; 1.17.4.1 [EC: 1.17.4.1]; 1.17.4.1 [EC: 1.17.4.1]; 1.17.4.1 [EC: 1.17.4.1]

Predicted SEED Role

"Ribonucleotide reductase of class Ib (aerobic), alpha subunit (EC 1.17.4.1)" in subsystem Ribonucleotide reduction (EC 1.17.4.1)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (8/8 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (7/7 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
• adenosine deoxyribonucleotides de novo biosynthesis II (4/4 steps found)
• guanosine deoxyribonucleotides de novo biosynthesis II (4/4 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• adenosine deoxyribonucleotides de novo biosynthesis I (2/2 steps found)
• guanosine deoxyribonucleotides de novo biosynthesis I (2/2 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)

KEGG Metabolic Maps

• Glutathione metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.17.4.1

Use Curated BLAST to search for 1.17.4.1

Protein Sequence (714 amino acids)

>EX28DRAFT_2761 ribonucleoside-diphosphate reductase, alpha subunit (Enterobacter asburiae PDN3)
MATTTAERVIQATPDYHALNAMLNLYDWEGRIQFEKDREAVDAFFAAHVRPNSVTFASQN
ERLDYLVNEGYYDARTLTRYDRAFVVTLFERAHASGFRFQTFLGAWKYYTSYTLKTFDGK
RYLESFEDRTVMVALTLAQGDEALAEQLTVEILSGRFQPATPTFLNCGKAQRGELVSCFL
LRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLED
AFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFKLA
KDNAEMALFSPYDIERIYGKAFGDVAISELYDELVADDRIRKKTINARDFFQRLAEIQFE
SGYPYIMFEDTVNRANPIAGRINMSNLCSEILQVNSASAYDENLDYADIGKDISCNLGSL
NIAHTMDSPDFGRTVETAIRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLAR
EGIAYGSPEGLDFTNLYFYTITWHALHTSMMLARERNQRFAGFEQSRYASGEYFSQYLEG
DWQPKTEKVRELFARAGITLPTREMWQQLCDDVIRYGIYNQNLQAVPPTGSISYINHATS
SIHPIVSKIEIRKEGKTGRVYYPAPFMTNENLALYQDAYEIGPEKIIDTYAEATKHVDQG
LSLTLFFPDTATTRDINRAQIYAWKKGIKTLYYIRLRQLALEGTEIEGCVSCAL