Protein Info for EX28DRAFT_2667 in Enterobacter asburiae PDN3

Annotation: phosphoheptose isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13580: SIS_2
  • amino acids 13 to 146 (134 residues), 105.7 bits, E=2.1e-34
• TIGR00441: phosphoheptose isomerase
  • amino acids 35 to 187 (153 residues), 278.7 bits, E=6.6e-88
• PF01380: SIS
  • amino acids 107 to 163 (57 residues), 30.4 bits, E=3.2e-11

Best Hits

Swiss-Prot: 98% identical to GMHA_SALCH: Phosphoheptose isomerase (gmhA) from Salmonella choleraesuis (strain SC-B67)

KEGG orthology group: K03271, phosphoheptose isomerase [EC: 5.-.-.-] (inferred from 100% identity to enc:ECL_01054)

MetaCyc: 97% identical to D-sedoheptulose 7-phosphate isomerase (Escherichia coli K-12 substr. MG1655)
RXN0-4301 [EC: 5.3.1.28]

Predicted SEED Role

"Phosphoheptose isomerase 1 (EC 5.3.1.-)" in subsystem Capsular heptose biosynthesis or LOS core oligosaccharide biosynthesis (EC 5.3.1.-)

MetaCyc Pathways

• ADP-L-glycero-β-D-manno-heptose biosynthesis (5/5 steps found)
• GDP-D-glycero-α-D-manno-heptose biosynthesis (1/4 steps found)

KEGG Metabolic Maps

• Biosynthesis of ansamycins
• Biosynthesis of steroids
• Carotenoid biosynthesis - General
• Galactose metabolism
• Inositol phosphate metabolism
• Lipopolysaccharide biosynthesis
• Pentose and glucuronate interconversions

Isozymes

Compare fitness of predicted isozymes for: 5.-.-.-, 5.3.1.-

Use Curated BLAST to search for 5.-.-.- or 5.3.1.- or 5.3.1.28

Protein Sequence (192 amino acids)

>EX28DRAFT_2667 phosphoheptose isomerase (Enterobacter asburiae PDN3)
MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAGGKVLSCGNGGSHCDAM
HFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGYDHIFSRYVEAVGREGDVLLGIST
SGNSGNVIKAIAAAREKGMKVITLTGKDGGKMDGTADVEIRVPHFGYADRIQEIHIKVIH
ILIQLIEKEMVK