Protein Info for EX28DRAFT_2602 in Enterobacter asburiae PDN3

Annotation: Putative hemolysin

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 574 transmembrane" amino acids 197 to 215 (19 residues), see Phobius details PF19576: Acyltransf_2" amino acids 24 to 258 (235 residues), 79.8 bits, see alignment E=2.5e-26 PF01553: Acyltransferase" amino acids 65 to 191 (127 residues), 46.3 bits, see alignment E=5.5e-16 PF13444: Acetyltransf_5" amino acids 322 to 423 (102 residues), 86.2 bits, see alignment E=3.4e-28

Best Hits

KEGG orthology group: None (inferred from 96% identity to enc:ECL_01127)

MetaCyc: 61% identical to ornithine lipid synthase (Serratia proteamaculans 568)
RXN-12431 [EC: 2.3.2.30]; RXN-12432 [EC: 2.3.2.30, 2.3.1.270]

Predicted SEED Role

"Putative hemolysin"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.270 or 2.3.2.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (574 amino acids)

>EX28DRAFT_2602 Putative hemolysin (Enterobacter asburiae PDN3)
MFSLDSVLDDLWPQARPAPWQKSLLKRLFYEDEFQQFAAAHRHLKGLDMVEQVLEHLDIL
CTVSARDLEQIPEHGPLVIIANHPTGTLDGLALMYAVSRVRRDVKVVTNRMLTHLEPLSS
LFIPVDNMGGRTAKSSLVQMEQHLQNAGVLIFFPAGEVSRPTRKGIRDKKWHSGFIKLAS
KLRAPLLPVHIQAHNSLLFYASTLVSPTLSMLLLMQQMFRRRHSQLPIKIGQQIAWHHWH
SATLSSREMAEQCRQHVMRLGKGVPGVFKTQCAIARPEDRATLKRALAQAECLGNTSDGK
TIYIWQRNGQEDAPLLRELGRLREIAFRAVEEGSGKRRDTDCYDDDYLHLILWDDEDLEI
VGAYRFMPTARQVERRGLEGLYSYSLFHYDDKMQDVLEHGIELGRSFIQPRYWGRRGLDY
LWSGIGAYLARYPNYRYLFGPVSISGGLPPAARDLLVAFYRLWFPATHPLAASRQPYPAS
LPDVLAQFGGVDYVDDLTKLKSLLGNLGCGIPPLYKQYSELCEPGGVQFIDFGSDPAFND
CVDGLVLVDLCYLKANRYQRYIEAHLIPSPQPSP