Protein Info for EX28DRAFT_2586 in Enterobacter asburiae PDN3

Annotation: diguanylate cyclase (GGDEF) domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 367 transmembrane" amino acids 42 to 68 (27 residues), see Phobius details amino acids 103 to 126 (24 residues), see Phobius details amino acids 138 to 158 (21 residues), see Phobius details amino acids 170 to 189 (20 residues), see Phobius details PF05230: MASE2" amino acids 36 to 124 (89 residues), 87.2 bits, see alignment E=6.4e-29 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 202 to 366 (165 residues), 198.3 bits, see alignment E=3.8e-63 PF00990: GGDEF" amino acids 207 to 361 (155 residues), 156.8 bits, see alignment E=4.2e-50

Best Hits

Swiss-Prot: 64% identical to DGCC_ECOL6: Probable diguanylate cyclase DgcC (dgcC) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: None (inferred from 77% identity to enc:ECL_01143)

MetaCyc: 64% identical to diguanylate cyclase DgcC (Escherichia coli K-12 substr. MG1655)
Diguanylate kinase. [EC: 2.7.7.65]

Predicted SEED Role

"Protein YaiC"

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.65

Use Curated BLAST to search for 2.7.7.65

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (367 amino acids)

>EX28DRAFT_2586 diguanylate cyclase (GGDEF) domain (Enterobacter asburiae PDN3)
MMNDETYYNKKRIREEWNLTLSQDDPHRAGIQFARRVRLARAVGLAAMFFPVAGMLVTHF
LPGGWWLLLVGWSFVWPHFAWQLSCRAASPHQQEIFNLKIDAIIAGLWIGMMGMNALPTA
ALVMMVGMNMMGSGGCRLFIPGIALTLLSALVTLPLAGKVVIFNPDPIEWSLTLPVLMLY
PMLFAWLSHRTAVRLAEHKQRLEMMSTRDGMTGVFNRRHWEMLLRNEFEHCRRDHSTATI
LLIDIDHFKNINDTWGHDVGDEAIIAITRQLQRSVRSGDAIGRFGGDEFAVIMSQTAAES
AIAVMSRLHERLEKLTLPCAPKEALRISVGVAPWGPQFGHYREWLKAADVALYRAKNAGR
GRTEVAA