Protein Info for EX28DRAFT_2556 in Enterobacter asburiae PDN3

Annotation: diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 367 PF14437: MafB19-deam" amino acids 1 to 143 (143 residues), 54.7 bits, see alignment E=1.5e-18 PF00383: dCMP_cyt_deam_1" amino acids 2 to 99 (98 residues), 77.1 bits, see alignment E=1.3e-25 TIGR00326: riboflavin biosynthesis protein RibD" amino acids 7 to 362 (356 residues), 503.7 bits, see alignment E=2.7e-155 TIGR00227: riboflavin-specific deaminase C-terminal domain" amino acids 146 to 365 (220 residues), 258.2 bits, see alignment E=5.5e-81 PF01872: RibD_C" amino acids 147 to 362 (216 residues), 201.1 bits, see alignment E=2.6e-63

Best Hits

Swiss-Prot: 88% identical to RIBD_ECOLI: Riboflavin biosynthesis protein RibD (ribD) from Escherichia coli (strain K12)

KEGG orthology group: K11752, diaminohydroxyphosphoribosylaminopyrimidine deaminase / 5-amino-6-(5-phosphoribosylamino)uracil reductase [EC: 1.1.1.193 3.5.4.26] (inferred from 97% identity to enc:ECL_01172)

MetaCyc: 88% identical to fused diaminohydroxyphosphoribosylaminopyrimidine deaminase / 5-amino-6-(5-phosphoribosylamino)uracil reductase (Escherichia coli K-12 substr. MG1655)
Diaminohydroxyphosphoribosylaminopyrimidine deaminase. [EC: 3.5.4.26]; 5-amino-6-(5-phosphoribosylamino)uracil reductase. [EC: 3.5.4.26, 1.1.1.193]

Predicted SEED Role

"Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) / 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193)" in subsystem Riboflavin, FMN and FAD metabolism (EC 1.1.1.193, EC 3.5.4.26)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.193 or 3.5.4.26

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (367 amino acids)

>EX28DRAFT_2556 diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) (Enterobacter asburiae PDN3)
MQDEIYMARAMKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGFHYRAGEPHAEVHALRMAG
EKARGATAYVTLEPCSHHGRTPPCCEALIAAGVSRVVASMQDPNPQVAGRGLYRLQQEGI
DVSHGLMMQDAEAINKGFLKRMRTGFPYIQLKLGASLDGRTAMANGESQWITSPQARRDV
QRLRAQSHAILTSSETVLADDPVMTVRWDELNADTQALYPQENLRQPLRIIIDSQNRVTP
AHRIVQQPGETWIARTKEDTREWPEGVRSIMVPEHNGHLDLVVLMMLLGKQQVNSIWVEA
GPTLAGALLQAGLVDELIVYVAPKLLGHDARGLFVLPGLEKLADAPQLKFSEIRPVGPDV
CLHLTTA