Protein Info for EX28DRAFT_2551 in Enterobacter asburiae PDN3

Annotation: 1-deoxy-D-xylulose-5-phosphate synthase (EC 2.2.1.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00204: 1-deoxy-D-xylulose-5-phosphate synthase
  • amino acids 11 to 619 (609 residues), 1067.2 bits, E=0
• PF13292: DXP_synthase_N
  • amino acids 13 to 282 (270 residues), 391.3 bits, E=5.9e-121
• PF00676: E1_dh
  • amino acids 123 to 186 (64 residues), 24.9 bits, E=2.5e-09
• PF02775: TPP_enzyme_C
  • amino acids 126 to 183 (58 residues), 22.9 bits, 1.7e-08
• PF02779: Transket_pyr
  • amino acids 319 to 480 (162 residues), 160.1 bits, E=1.1e-50
• PF02780: Transketolase_C
  • amino acids 495 to 611 (117 residues), 91.2 bits, E=1.3e-29

Best Hits

Swiss-Prot: 96% identical to DXS_ENT38: 1-deoxy-D-xylulose-5-phosphate synthase (dxs) from Enterobacter sp. (strain 638)

KEGG orthology group: K01662, 1-deoxy-D-xylulose-5-phosphate synthase [EC: 2.2.1.7] (inferred from 98% identity to enc:ECL_01177)

MetaCyc: 94% identical to 1-deoxy-D-xylulose-5-phosphate synthase (Escherichia coli K-12 substr. MG1655)
1-deoxy-D-xylulose-5-phosphate synthase. [EC: 2.2.1.7]

Predicted SEED Role

"1-deoxy-D-xylulose 5-phosphate synthase (EC 2.2.1.7)" in subsystem Isoprenoid Biosynthesis or Pyridoxin (Vitamin B6) Biosynthesis or Thiamin biosynthesis (EC 2.2.1.7)

MetaCyc Pathways

• superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (12/12 steps found)
• superpathway of thiamine diphosphate biosynthesis I (10/10 steps found)
• methylerythritol phosphate pathway I (9/9 steps found)
• methylerythritol phosphate pathway II (9/9 steps found)
• superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) (11/12 steps found)
• pyridoxal 5'-phosphate biosynthesis I (7/7 steps found)
• isoprene biosynthesis I (9/10 steps found)
• thiazole component of thiamine diphosphate biosynthesis I (6/6 steps found)
• taxadiene biosynthesis (engineered) (11/13 steps found)
• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• thiazole component of thiamine diphosphate biosynthesis II (5/7 steps found)
• superpathway of ergosterol biosynthesis II (11/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Terpenoid biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.2.1.7

Protein Sequence (620 amino acids)

>EX28DRAFT_2551 1-deoxy-D-xylulose-5-phosphate synthase (EC 2.2.1.7) (Enterobacter asburiae PDN3)
MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVSRSSGHFASGLGTVELT
VALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKGGLHPFPWRGESEYDVLSV
GHSSTSISAGIGIAVAAEKENKQRRTVCVIGDGAITAGMAFEAMNHAGDIKPDMLVILND
NEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSGVPPIKELLKRTEEHIKGMVVPG
TLFEELGFNYIGPVDGHDVLGLVTTLKNMRDLKGPQFLHIMTKKGRGYEPAEKDPITFHA
VPKFDPSSGCLPKSSGGMPSYSKIFGDWLCETAAKDNKLMAVTPAMREGSGMVEFSRKYP
DQYFDVAIAEQHAVTFAAGLAIGGYKPVVAIYSTFLQRAYDQVIHDVAIQKLPVLFAIDR
AGIVGADGQTHQGAFDLSFLRCIPDMVVMTPSDENECRQMLFTGYHYQDGPSAVRYPRGN
AVGVELQPLEKLPIGKGLVKRRGEKVAILNFGTLMPEAAKAAEALNATLVDMRFVKPLDE
SLILSMAETHDVLVTLEENAIMGGAGSGVNEVLMANRKAVPVLNLGLPDHFIPQGTQDEA
RAAIGLDAAGIEAKIRAWLD