Protein Info for EX28DRAFT_2548 in Enterobacter asburiae PDN3

Annotation: tRNA sulfurtransferase ThiI/thiazole biosynthesis domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 482 TIGR00342: tRNA sulfurtransferase ThiI" amino acids 4 to 377 (374 residues), 473.9 bits, see alignment E=3.6e-146 PF22025: ThiI_fer" amino acids 9 to 76 (68 residues), 38.4 bits, see alignment E=2e-13 PF02926: THUMP" amino acids 85 to 161 (77 residues), 55 bits, see alignment E=1.4e-18 PF02568: ThiI" amino acids 175 to 369 (195 residues), 269.7 bits, see alignment E=2.1e-84 TIGR04271: thiazole biosynthesis domain" amino acids 382 to 482 (101 residues), 136.2 bits, see alignment E=3.6e-44

Best Hits

Swiss-Prot: 93% identical to THII_SALPA: tRNA sulfurtransferase (thiI) from Salmonella paratyphi A (strain ATCC 9150 / SARB42)

KEGG orthology group: K03151, thiamine biosynthesis protein ThiI (inferred from 97% identity to enc:ECL_01180)

MetaCyc: 92% identical to tRNA uridine 4-sulfurtransferase (Escherichia coli K-12 substr. MG1655)
tRNA sulfurtransferase. [EC: 2.8.1.4]; 2.8.1.- [EC: 2.8.1.4]

Predicted SEED Role

"tRNA S(4)U 4-thiouridine synthase (former ThiI) / Rhodanese-like domain required for thiamine synthesis" in subsystem Thiamin biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (482 amino acids)

>EX28DRAFT_2548 tRNA sulfurtransferase ThiI/thiazole biosynthesis domain (Enterobacter asburiae PDN3)
MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVVRHWDHVEVRAKDENKR
QDIRDALTRIPGIHHILEVEDVPFSDMHDIFEKALVQYRDQIEGKTFCVRVKRRGKHEFS
SIEVERYVGGGLNQHVETARVRLTNPDVTVNLEIENDRLLLVKGRYEGIGGFPIGTQEDV
LSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVRQVAHYLWNRFGSSHRVRFV
AINFEPVVGEILEKVDDGQMGVVLKRMMVRAASKVAERYGVQALVTGEALGQVSSQTLTN
LRLIDNVSDTLILRPLISHDKEHIIDLAREIGTEDFARTMPEYCGVISKSPTVKAVKAKI
EAEEENFDFSILEKVVEEASNIDIREIAQQTEQEVVEVETVSGFGASDVIVDIRSIDEQD
ASPLNVEGVEVVSLPFYKLSTKFGDLDKSKTYLLWCERGVMSRLQALYLREQGFANVKVY
RP