Protein Info for EX28DRAFT_2529 in Enterobacter asburiae PDN3

Annotation: Parvulin-like peptidyl-prolyl isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 623 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details PF13624: SurA_N_3" amino acids 1 to 165 (165 residues), 180.4 bits, see alignment E=5.8e-57 PF13623: SurA_N_2" amino acids 2 to 141 (140 residues), 122.8 bits, see alignment E=2.6e-39 PF13145: Rotamase_2" amino acids 248 to 369 (122 residues), 82 bits, see alignment E=1.6e-26 PF00639: Rotamase" amino acids 275 to 355 (81 residues), 51 bits, see alignment E=6.1e-17 PF13616: Rotamase_3" amino acids 276 to 355 (80 residues), 45.3 bits, see alignment E=3.1e-15

Best Hits

Swiss-Prot: 84% identical to PPID_ECOL6: Peptidyl-prolyl cis-trans isomerase D (ppiD) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K03770, peptidyl-prolyl cis-trans isomerase D [EC: 5.2.1.8] (inferred from 96% identity to enc:ECL_01199)

Predicted SEED Role

"Peptidyl-prolyl cis-trans isomerase PpiD (EC 5.2.1.8)" (EC 5.2.1.8)

Isozymes

Compare fitness of predicted isozymes for: 5.2.1.8

Use Curated BLAST to search for 5.2.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (623 amino acids)

>EX28DRAFT_2529 Parvulin-like peptidyl-prolyl isomerase (Enterobacter asburiae PDN3)
MMDSLRTAANSLVLKIIFGIIIVSFILTGVSSYLIGGGANYAAKVNGQEISRGQFENAFA
GERNRMQQQLGDQFSELAANEGYMKTLRQQTLNRLIDEALLDQYAKKLGLGISDEQVKKA
IFSTQAFQSNGKFDNARYNSIVNQMGMTADQYAQALRNQLTTQQLINAVVGTDFMLKGET
DELAALVAQQRVVREATIDVNALAAKQQVSDADVNAYYEQNKNSFVSPEQFRVSYIKLDA
AALQETASDAEIQSYYDQHQDQFTQPQRNRYSVIQTKTEADAKAALDELNKGADFATVAK
AKSTDIISAKNGGDMGWLEDATTPDELKNAGLKEKGQLSGVIKSSVGFLVVRLDDITAAK
TKPLADVRDDIAAKVKQEKALDAYYALQQKVSDAASNDNESLAGAEQAAGVKAVETGWFG
RDNLPEELNFKPVSDAIFNGGLVGENGTPGSNSDIITVDGDRAFVLRVSEHKPEAVKPLA
EVKDQVVAQVKHNKAEQQAKLDAEKILSDLKAGKEDALKAAGLSFGEAKTLSRTGQDPIS
QAAFGLTLPAKDKPSFGTTTDTQGNVVLLALDEVKAGTLPEAQKKAMVQGITQNNAQIAF
EAMMSNLRKEAKIKLGDVMTQQQ